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QIAamp PowerFecal Pro DNA Kit

For the isolation of microbial DNA from stool and gut samples
  • Efficient lysis of bacteria and fungi from stool and gut samples
  • Up to 20-fold more DNA compared to alternative methods
  • Yield higher alpha diversity in sequencing compared to other methods
  • Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications
  • Automate workflows on the QIAcube

The QIAamp PowerFecal DNA Kit removes tough inhibitors from stool, gut and biosolid samples and provides clean, ready-to-use DNA for any downstream application. With the included Inhibitor Removal Technology, inhibitors from digested food, heme from lysed red blood cells in stool and other PCR inhibitors are eliminated leaving only high-quality DNA ready for downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.


The QIAamp PowerFecal DNA Kit can be used interchangeably with the DNeasy PowerSoil Kit. Both kits can be used to isolate DNA from soil, environmental, stool, gut and biosolid samples.


Isolation of DNA using the QIAamp PowerFecal DNA Kit can be automated on the QIAcube Connect. Want to try this solution for the first time? Request a quote for a trial kit.

QIAamp PowerFecal DNA Kit was formerly sold by MO BIO as PowerFecal DNA Isolation Kit.

Buy Products

Cat No./ID: 12830-50
QIAamp PowerFecal DNA Kit
For the isolation of DNA from stool, gut material and biosolids
Cat No./ID: 12830-S
QIAamp PowerFecal DNA Sample (2)
Isolate DNA from stool, gut material and biosolids.
Cat No./ID: 51804
QIAamp PowerFecal Pro DNA Kit
For the isolation of microbial DNA from stool and gut samples

QIAamp PowerFecal Pro DNA Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

Performance

Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: ‘Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.’).

Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: ‘The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.’).

Principle
The streamlined Inhibitor Removal Technology® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).
Procedure
Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thorough homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to remove inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted, ready for downstream applications.
Applications

Tools to complete your workflow:


  1. Sample disruption: PowerLyzer 24 Homogenizer
  2. Sample extraction: QIAamp PowerFecal DNA Kit
  3. Library preparation: QIAseq 1-step Amplicon Kit (16S rRNA gene sequencing) or QIAseq FX DNA Library Kit (metagenomic shotgun sequencing)
  4. Analysis: QIAGEN Microbial Genomics Pro Suite (request a demo)


Specifications

Features
Specifications
磁珠大小 0.7 mm garnet
结合能力 Up to 20 µg per filter
规格 Silica spin filter tubes
处理 Bead beating
样本大小 0.25 g
样本类型 Stool, gut material, biosolids, soil
储存温度 Store at room temperature (15-30°C)
通量 1-24 samples
每次运行或制备时间 30 minutes

Product Resources

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试剂盒操作手册 (1)
For the isolation of microbial genomic DNA from stool and gut samples
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在线讲座 (1)
This webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples.
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