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QIAamp Fast DNA Stool Mini Kit

用于从粪便样本中分离gDNA
  • 快速、高效的纯化实验方案
  • 不使用危险化学品
  • 可在QIAcube全自动核酸纯化仪上自动操作
  • 高效去除PCR抑制剂
  • 灵敏度高,适合多种下游应用
QIAamp Fast DNA Stool Mini Kit可从新鲜或冷冻的人类粪便样本中快速纯化高品质的人类或细菌基因组DNA。新型InhibitEX Buffer代替了繁琐的抑制剂去除片,可高效去除粪便样本中的PCR抑制剂。该试剂盒实验步骤简化,使您的工作流程更加高效,缩短抽提DNA的手动操作时间。QIAamp Fast DNA Stool Mini Kit可用于微量离心机,或在QIAcube全自动核酸纯化仪上自动纯化DNA。

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Cat No./ID: 19593
Inhibitex Buffer (140/250)
¥779.00
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Inhibitex Buffer (140 ml)
Cat No./ID: 51604
QIAamp Fast DNA Stool Mini Kit (50)
For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX Buffer, wash and elution buffers, Collection Tubes (2 ml)

QIAamp Fast DNA Stool Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
High sensitivity in qPCR.
DNA from a stool sample was purified using the QIAamp Fast DNA Stool Mini Kit and solutions from 2 alternative suppliers. Equal volumes of resulting eluates with isolated DNA were used to amplify the human Alu element. Represented in the amplification plot are the means of 2 replicates. The QIAamp Fast DNA Stool Mini Kit produced the lowest CT values.
9
Efficient depletion of PCR inhibitors.
DNA was isolated from 6 stool samples using either the QIAamp Fast DNA Stool Mini Kit or another standard column method. CT values were measured with an inhibitor-sensitive PCR system. In most samples using the standard purification method the target could not be amplified due to PCR inhibition. The QIAamp Fast DNA Stool Mini Kit efficiently removed inhibitors and enabled amplification with high sensitivity.
9
Streamlined procedure of the QIAamp Fast DNA Stool Mini Kit.
The novel InhibitEX Buffer included in the QIAamp Fast DNA Stool Mini Kit reduces handling time of samples and streamlines the purification to as little as 25 minutes.
9
Superior DNA yields compared to similar kits.
DNA was purified from 7 stool samples using the QIAamp Fast DNA Stool Mini Kit and solutions from 2 alternative suppliers. Yields were measured by UV spectrophotometry. Yields were invariably highest with the QIAamp Fast DNA Stool Mini Kit.
Performance
产物DNA产量高,质量出色,适用于敏感的下游应用

QIAamp Fast DNA Stool Mini Kit实验方案经过优化,可确保从粪便样本中获得搞产量DNA,且花费时间少于其他常用方法。此外,该试剂盒不含危险化学品,确保实验室和人员安全。QIAamp Fast DNA Stool Mini Kit的常规产量为5–50 µg,产量高于比其他供应商的同类产品(参见Superior yields compared to similar purification kits)。

QIAamp Fast DNA Stool Mini Kit纯化获得的DNA质量出色,能够在下游的定量real-time PCR中获得灵敏可靠的结果。相较于其他供应商的同类产品获得更低的CT值(Higher sensitivity in qPCR)。

高效去除PCR抑制剂

粪便样本的PCR抑制剂含量高,含有复杂多糖、胆酸盐、脂类和尿酸等。即便情况较好时,这些抑制剂会使核酸难以扩增。最差的情况,抑制剂会完全抑制PCR信号。因此,对于粪便样本分析,完全去除此类抑制剂意义重大。QIAamp Fast DNA Stool Mini Kit中新型的InhibitEx Buffer能够高效的去除PCR抑制剂,实现DNA的可靠检测(参见Efficient depletion of PCR inhibitors)。
Principle
DNA特异性结合到QIAamp硅胶模上,其他污染物流出。无需酚-氯仿抽提。PCR抑制剂在InhibitEX Buffer中被去除。产物是纯度极高的DNA,可即用于下游应用;整个过程仅需25分钟。
Procedure
QIAamp Fast DNA Stool Mini Kit采用快速离心柱技术和高效的实验方案,简化了粪便样本的DNA分离步骤(参见Streamlined procedure of the QIAamp Fast DNA Stool Mini Kit),将DNA分离所需的时间缩短至25分钟。

配方独特的InhibitEX Buffer能够高效从将DNA与PCR抑制剂分离,该该缓冲液为液体,因此比去除抑制剂的片剂或粉状物更加便利。您无需单独加入抑制剂去除剂,手动操作时间缩短。QIAamp Fast DNA Stool Mini Kit可在QIAcube全自动核酸纯化仪上自动操作。

该试剂盒配套快速实验方案,能够高效分离人类DNA,以及微生物DNA用于致病病原检测。将粪便样本混悬于InhibitEX Buffer,DNA与抑制剂分离开。之后进行快速裂解,DNA结合到QIAamp硅胶模上。残留的抑制剂和污染物在洗涤步骤去除,最后将纯化的完整DNA从柱上洗脱。
Applications
QIAamp Fast DNA Stool Mini Kit能够从新鲜或冷冻的粪便样本中分离人类或细菌基因组DNA。

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