QIAseq Investigator Panels

Dedicated QIAseq panels for human identity investigations

Features

  • Maximize accuracy and confidence in your data with the inclusion of Unique Molecular Indices
  • UMIs allow you to track every molecule from start to finish
  • Utilize QIAseq Investigator panels on Illumina and Ion Torrent sequencers with platform-agnostic chemistry
  • Focus on the markers that you need in your case with our wide range of dedicated panels
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QIAseq Targeted DNA Panel (12)

Cat. No. / ID: 333502

Kit containing ALL reagents (except indexes) for targeted DNA sequencing; fixed panel for 12 samples; less than 100 genes
$1,337.00
Panel
QIAseq Targeted DNA Panel
QIAseq Targeted DNA Custom Panel
Samples
12
96
 CONFIGURE AT GeneGlobe
QIAseq Investigator Panels are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Developed in partnership with leading human identity labs around the world, QIAseq Investigator panels are designed to pick up where conventional STR/capillary testing cannot resolve a case. There are separate, dedicated panels for common human identify questions like biogeographical ancestry prediction, missing persons identification, or paternity testing. Focus your sequencing and data analysis efforts only on the markers of interest in your own case and increase your chances of success with QIAseq.

QIAseq Investigator Missing Persons SNP panel CDHS-15861Z-2897
Highly discriminating identity SNP panel enabling kinship confirmation for challenging missing persons samples using immediate and extended family references.

QIAseq Investigator ID SNP panel CDHS-14055Z-277
Identity SNP panel comprising specially selected kinship SNPs designed for use in kinship and other identification applications.

QIAseq Investigator Global Ancestry SNP panel CDHS-12534Z-204
For accurate and reliable biogeographical ancestry inference using SNPforID AIMs SNPs.

QIAseq Investigator Middle East Ancestry SNP panel CDHS-12533Z-169
Supplementary SNP panel for increased accuracy in biogeographical ancestry inference from putative middle east samples.

QIAseq Investigator Microhaplotype panel CDHS-15034Z-170
Microhaplotype identity panel for high levels of discrimination.

QIAseq Human Mitochondria panel DHS-105Z
Whole mitochondrial genome sequencing in human identity applications.

QIAseq Investigator Human Mitochondria Control Region panel CDHS-13743Z-27
Control Region sequencing for the mitochondrial genome

Do you need a specific customized NGS panel for your human identity investigations not covered by the Investigator panels? Our easy-to-use custom builder lets you design a sequencing panel targeting your regions of interest in just a few clicks.

Performance

  • Accuracy: Innovative digital sequencing (incorporating molecular barcodes) eliminates PCR duplication and amplification artifacts to detect low-frequency variants with high confidence (see figure Principle of molecular barcodes).
  • Specificity: The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results.
  • Uniformity: The QIAseq Targeted DNA Panel workflow has been optimized to deliver highly uniform sequencing results, to ensure sequencing capacity is utilized very efficiently (see figure Uniformity).
  • Sensitivity: Digital DNA sequencing approach is optimized to deliver high confidence in calling low-frequency DNA variants. Over 90% sensitivity for 1% NA12878 SNP and indel on typical coding region with false positive less than 15 per mega base region when variants are detected with tiled primer design to cover complete coding region of each gene.
  • Universality: The chemistry used in the QIAseq targeted DNA panels and workflow is compatible with both regular and GC-rich genomic regions, allowing one to achieve 100% coverage of genes rich in GC content such as CEBPA and CCND1 (see figure: coverage of GC-rich genomic regions)
  • Flexibility: The QIAseq targeted DNA panels offer a high degree of flexibility in content and sample multiplexing. Several cataloged panels have been developed for a wide range of applications. One can also build a custom panel for a specific content, or extend the contents of an existing cataloged panel; Up to 384 samples can be multiplexed using the QIAseq indexes.

Principle

PCR duplicates are a major issue in targeted DNA sequencing, since, through PCR amplification, they turn unique DNA molecules into identical DNA molecules that cannot be distinguished from each other. In addition, errors from PCR amplification and sequencing process may also be present in final reads that lead to false positive variants in sequencing results. This, in turn, results in the inability to confidently call DNA variants present at low frequencies in the starting DNA material. To overcome the issue of PCR duplicates and amplification artifacts, the QIAseq Targeted DNA Panels use digital sequencing by incorporating molecular barcodes into the starting DNA material before any amplification takes place, thereby preserving the uniqueness of the starting DNA molecules and overcoming the issues of PCR duplicates, false positives and library bias.

Procedure

The entire workflow of the QIAseq targeted DNA panels to go from extracted DNA to sequencing-ready libraries can be completed in 9 hours (see figure Workflow). Extracted DNA is fragmented, genomic targets are molecularly barcoded and enriched, and libraries are constructed. Sequencing files can be fed into the QIAseq pipeline, a cloud-based data analysis pipeline, which will filter, map and align reads, as well as count unique molecular barcodes associated with targeted genomic regions, and call variants with a barcode-aware algorithm. This data can then be fed into IVA or QCI for interpretation.

Applications

The QIAseq targeted DNA panels can be used to call a variety of DNA variants from a wide range of sample types for numerous applications.

DNA variants:

  • SNVs
  • Small indels
  • CNVs

Sample types:

  • Plasma/serum
  • Fresh or frozen tissue

Applications:

  • Human identity and paternity testing
  • Profiling of DNA variants
  • Examination of variants in mitochondrial DNA

Supporting data and figures

Resources

Brochures & Guides (6)

Next-generation sequencing using QIAGEN’s QIAseq – a powerful tool for human identification


State-of-the-art technologies to fast-track and streamline NGS workflows
Advanced sample collection, automation and STR solutions for kinship testing
Kit Handbooks (2)
For ultrasensitive targeted next-generation sequencing (NGS) of DNA for Illumina NGS systems
For ultrasensitive targeted next-generation sequencing (NGS) of DNA for Ion Torrent NGS systems