QIAamp DNA Investigator Kit
For purification of DNA from forensic and human identity samples
- Rapid purification of high-quality, ready-to-use DNA
- Consistent, high yields
- Complete removal of contaminants and inhibitors
Cat. No. / ID: 56504
The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. Purification of DNA using the QIAamp DNA Investigator Kit can be automated on the QIAcube Connect.
The QIAamp DNA Investigator Kit is suited for DNA purification from trace samples (e.g., from crime scene); genotyping, including fingerprinting and paternity analysis; and routine analysis of reference samples.
After sample lysis, the simple QIAamp DNA Investigator procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes. DNA is eluted in Buffer ATE or water, and is immediately ready for use in amplification reactions or for storage at –20°C. The purified DNA is free of proteins, nucleases, and other inhibitors.
The QIAamp DNA Investigator Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl.
The procedure is designed to ensure that there is no sample-to-sample cross-contamination.
The QIAamp DNA Investigator Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes.
|Applications||Real-time PCR, STR analysis|
|Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein||Genomic DNA, mitochondrial DNA|
|Sample types||wide range of forensic and human-identity sample materials|
|Elution volume||20-100 µl|
|Time per run or per prep||less than 30 minutes|
|Yield||< 3 µg|
The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.
Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.
The composition of Buffer ATE is:
- 10 mM Tris-Cl pH 8.3
- 0.1 mM EDTA
- 0.04% NaN3 (sodium-azide)
It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.
Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.
Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.
Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.
Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).