Qproteome FFPE Tissue Kit

For isolation of full-length proteins from formalin-fixed tissues

Features

  • Standardized, quantitative extraction from FFPE tissue
  • Full-length proteins
  • Low sample requirements
Qproteome FFPE Tissue Kit (20)

Cat. No. / ID: 37623

For 20 protein preparations from formalin-fixed, paraffin-embedded tissue samples: Extraction Buffer, Collection Tubes, Collection Tube Sealing Clips
The Qproteome FFPE Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse the crosslinking process of fomalin fixation and deliver full-length proteins ready for subsequent analysis.

Performance

The Qproteome FFPE Tissue Kit delivers  efficient protein extraction from FFPE tissue using a standardized protocol for reproducible protein yields (see figure " Standardized and reproducible protein extraction"). Using the Qproteome FFPE Tissue Kit, protein  yields are comparable to frozen material, enabling, for example, comparison between different tissues for identification of disease-specific markers (see figure " tissue-specific biomarker identification").

 

See figures

Principle

As the standard method for histological tissue preparation, millions of formalin-fixed paraffin-embedded (FFPE) samples are classified and archived each year. Previously, their analysis was limited to histochemical techniques. The Qproteome FFPE Tissue Kit opens this vast, highly valuable resource up to proteomic and protein expression analysis by enabling efficient extraction of full-length proteins suitable for western blotting, protein arrays, and mass spectrometry. For the very first time, proteins or pathway of interest can be investigated in archived FFPE tissues. Probing blots or arrays with multiple antibodies enables simultaneous investigation of proteins, providing a detailed picture of multiple protein expression profiles, for example, in signaling transduction cascades.

Formalin fixation creates chemical crosslinks, stabilizing samples for histological analysis, but rendering any subsequent protein separation (e.g., by SDS-PAGE) impossible. The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse the crosslinking process and deliver full-length proteins ready for analysis by western blotting or protein array, with subsequent immunodetection (see figure “Compare FFPE Tissue Samples at the Protein Level”).

Procedure

Sections are deparaffinized and incubated in an Extraction Buffer. After centrifugation, extracted proteins are recovered in the supernatant. Recovered proteins can be mixed directly with sample buffer and loaded onto an SDS-PAGE gel, just like conventional protein samples. No extra expensive equipment or additional processing is required.

Applications

The Qproteome FFPE Tissue Kit offers a unique tool for analysis of samples whose pathological status has been determined, but whose precise protein makeup has until now remained inaccessible. Such samples — for example, taken over a course of treatment or from different groups of probands — offer a rich source of information for comparative proteomics and biomarker studies.

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsSDS-PAGE, Western-Blot, mass spectrometry
bindingcapacityyield20–80 µg
startmaterialFormalin-fixed tissues
samplesize2–3 x 100 mm2 per prep
fractionsisolatedOne fraction

Resources

Brochures & Guides (2)
Sample to Insight solutions for successful molecular analysis
Critical factors for molecular analysis of FFPE samples
Kit Handbooks (1)
Additional Resources (1)