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RNeasy PowerClean Pro CleanUp Kit

For the removal of inhibitors from purified RNA in just 7 minutes
  • Highly pure RNA from previously isolated RNA and efficient removal of a variety of inhibitors
  • Fast and easy cleanup of RNA with a 7-minute protocol
  • High-quality RNA ready for downstream PCR applications

The RNeasy PowerClean Pro CleanUp Kit is the only commercially available kit to purify previously isolated RNA. The kit works well on RNA isolated from virtually any sample source, including problematic soils, samples spiked with commercial humic acids, as well as archived and previously isolated RNA samples.

PCR-inhibiting substances may discolor starting RNA an amber to brown color, indicating the presence of humics or polyphenols, although some inhibitors are colorless. The RNeasy PowerClean Pro CleanUp Kit will remove all colors and inhibitors including heme, polysaccharides, polyphenols, fluvic acids and dyes, leaving only RNA of the highest purity. Pure RNA is then ready for successful RT-PCR amplification and other downstream applications.

The backbone of the kit is the Inhibitor Removal Technology, which selectively removes inhibitors from the RNA solution. RNA is captured on a silica spin column membrane to be washed and eluted.

The RNeasy PowerClean Pro CleanUp Kit was previously sold by MO BIO as the PowerClean Pro RNA Cleanup Kit.

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Cat No./ID: 13997-50
RNeasy PowerClean Pro Cleanup Kit (50)
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For the removal of inhibitors from purified RNA in just 7 minutes
The RNeasy PowerClean Pro CleanUp Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

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Figure 1. More RNA is visualized following clean up.
RNA visualized on the gels had similar quality, amount and RNA molecular weight between the starting sample and those following clean up. Humic acids in the starting sample absorbed at A230-320. Cleanup increased 260/280 and 260/230 ratios, consistent with pure RNA. Concentration of RNA following clean up decreased to an average of 129.93 ng/µl. Qubit® PicoGreen® Assay (not shown) confirmed quantification. As humic acids falsely inflated concentrations due to absorbance at A230-320, a decrease in concentration is expected from clean, purified RNA.
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Figure 2. More RNA, regardless of dilution.
Examination of RNA described in Figure 1 using RT-qPCR and a Bacillus 16S assay compared 1 µl of undiluted RNA against a 1:10 dilution. Undiluted, purified samples were successfully amplified and approximately 3 cycles existed between the cleaned undiluted and 1:10 dilution, showing lack of inhibitors. Starting samples still contained inhibitors, as shown by a failure to amplify either the undiluted or 1:10 dilutions.
Performance


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Specifications

Features
Specifications
Binding capacity Up to 20 µg per prep
Format Silica Spin Filters
Sample size All RNA sizesUp to 150 µl of purified RNA
Sample types Previously purified RNA
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 7 minutes

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