TissueLyser LT

For low- to medium-throughput sample disruption for molecular analysis

Features

  • Simultaneous disruption of up to 12 samples
  • Compact instrument with small footprint
  • Coolable adapter to prevent biomolecule degradation
  • Reproducible results with all sample types
  • Compatible with all laboratory workflows
undefined
TissueLyser LT

Cat. No. / ID: 85600

Compact bead mill, 100–240 V AC, 50–60 Hz; requires the TissueLyser LT Adapter, 12-Tube (available separately)
R$43,274.00
InstrumentAccessories
TissueLyser LT
TissueLyser LT Adapter
Add to cart
The TissueLyser LT is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The TissueLyser LT is a small bead mill which provides fast, effective disruption of up to 12 samples at the same time. This throughput matches that of the QIAcube, which automates sample preparation using trusted QIAGEN spin-column kits. Simultaneous disruption and homogenization is achieved through high-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads. The TissueLyser LT must be used in combination with the coolable TissueLyser LT Adapter, which holds tubes during the disruption process. Additional accessories include beads and bead dispensers.

Explore the virtual demo to learn more about the TissueLyser LT.

Performance

The TissueLyser LT provides effective disruption of human, animal, and plant tissues, bacteria, and yeast, allowing reproducible yields of DNA, RNA, and protein in subsequent purification procedures (see figure " Effective tissue disruption"). Even difficult-to-lyse tissues such as heart and brain can be processed by the TissueLyser LT. DNA, RNA, and protein remain intact after disruption (see figures " Pure RNA with high RIN values", " Intact protein suitable for all types of analysis", " High-quality DNA from animal tissues", " High-quality DNA from plant tissues", and " Intact RNA from plant tissues"), enabling reliable analysis in downstream applications.

Disruption with the TissueLyser LT is comparable to that achieved with the well-established TissueLyser II (see figures " Effective tissue disruption", " Intact protein suitable for all types of analysis", " High-quality DNA from animal tissues", and " High-quality DNA from plant tissues").

See figures

Principle

Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. The TissueLyser LT system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").

The TissueLyser LT is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser LT is fully compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems"), and also complements QIAGEN's range of automated solutions for medium-throughput sample preparation and analysis (see table "QIAGEN medium-throughput automation"). These include the QIAcube, which automates sample purification using QIAGEN spin-column kits, and the QIAxcel, which automates multicapillary gel electrophoresis of DNA and RNA.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified Sample typeQIAGEN kit
RNA Easy-to-lyse tissue

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits
RNA All types of tissue RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA Plant tissue RNeasy Plant Mini Kit
RNA Yeast RNeasy Kits
RNA Bacteria RNeasy Protect Bacteria Kits
microRNA All types of tissue miRNeasy Kits
DNA Human tissue QIAamp DNA Kits
DNA Animal tissue DNeasy Blood & Tissue Kits
Protein Tissue Qproteome Mammalian Protein Prep Kit
Phosphoprotein Tissue PhosphoProtein Purification Kit
Glycoprotein Tissue Qproteome Glycoprotein Kits
DNA and RNA Tissue AllPrep DNA/RNA Kits
DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Mini Kit

QIAGEN medium-throughput automation
InstrumentPurpose Throughput
QIAcube Purification of DNA, RNA, and protein Up to 12 samples per run
EZ1 Advanced Purification of DNA and RNA from human samples Up to 6 samples per run
EZ1 Advanced XL Purification of DNA and RNA from human samples Up to 14 samples per run
QIAxcel Electophoretic analysis of DNA fragments and RNA Up to 96 samples per run
QIAgility Reaction setup Up to 384 samples per run
Rotor-Gene Q Real-time PCR and high-resolution melting (HRM) analyses Up to 100 samples per run
PyroMark Q24 Methylation and mutation analyses Up to 24 samples per run

Procedure

The TissueLyser LT works in combination with the TissueLyser LT Adapter. Up to twelve 2 ml tubes, each containing a sample and a bead, are loaded into the adapter, which is securely fastened to the piston of the TissueLyser LT. The piston moves up and down rapidly, leading to simultaneous disruption and homogenization of the samples due to the beating and grinding action of the beads.

The TissueLyser LT has a small footprint of 15 cm x 27 cm, allowing installation in any laboratory. As each sample is safely sealed within its own tube, the TissueLyser LT is able to disrupt multiple samples without any risk of cross-contamination. If disrupting fresh or frozen samples, the TissueLyser LT Adapter can be precooled on dry ice to prevent degradation of nucleic acids and proteins (see figures " High-quality DNA from plant tissues" and " Intact RNA from plant tissues"). Tissues stored in Allprotect Tissue Reagent (to stabilize DNA, RNA, and protein) or in RNAprotect Tissue Reagent (to stabilize RNA) require no precooling of the adapter.

See figures

Applications

The ability to process up to 12 samples per run makes the TissueLyser LT the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. The TissueLyser LT enables fast and uniform disruption of animal and human tissues, plant tissues, bacteria, and yeast in the TissueLyser LT Adapter, which holds up to twelve 2 ml sample tubes. Sample purification can be performed manually, or can be automated using the QIAcube, QIAsymphony SP, QIAxtractor, EZ1 Advanced, EZ1 Advanced XL, or BioRobot, or BioSprint instruments.

Supporting data and figures

Specifications

FeaturesSpecifications
DimensionsWeight: Approx. 7 kg, Width: 150 mm, Depth: 270 mm, Height: 280 mm, The power supply of the TissueLyser LT is compatible with voltages of 100–240 V
Disruption principleHigh-speed shaking of samples in 2 ml microcentrifuge tubes with stainless steel or glass beads
Protocol/main application on this intrumentSample preparation/sample disruption
FeaturesSimultaneous disruption of up to 12 samples. Compact instrument with small footprint. Coolable adapter to prevent biomolecule degradation. Reproducible results with all sample types. Compatible with all laboratory workflows.
TechnologyBead Mill
Kits compatible with instrumentAll kits for purification of RNA/DNA/protein
Typical run time40 sec - 5 min, depending on protocol

FAQ

Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?

No, cooling with liquid nitrogen would make the sample tubes brittle, resulting in breakage of the tubes during the disruption process. Cooling the adapter and sample tubes on dry ice or in a freezer to approx. -80°C is sufficient to prevent the samples from thawing during the disruption process.

 

See:  TissueLyser Virtual Demo for a demonstration of cooling the samples on dry ice before using the TissueLyser LT.

FAQ ID -2549
How long does the sample disruption with the TissueLyser LT take?
Depending on the downstream application and the tissue, the disruption time typically takes 40 sec to 5 min. For more information about the different applications please refer to the TissueLyser LT Handbook.
FAQ ID -2550
Are there any accessories required for sample disruption with the TissueLyser LT?

Yes, disruption and homogenization are achieved through the beating and grinding effect of beads on the sample material as they are shaken together in 2 mL sample tubes. Sample tubes and different beads can be purchased at QIAGEN (see ordering information in the TissueLyser LT Handbook).

 

See:  TissueLyser Virtual Demo for a virtual demonstration of this process.

FAQ ID -2551
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

FAQ ID -374
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
The RNA yield can be improved by choosing a specialized RNeasy Kit, which is optimally adapted to the starting material (e.g., RNeasy Lipid Tissue Kits for brain tissue or RNeasy Fibrous Tissue Kit for heart).
FAQ ID -2555
What sample numbers can be processed on the TissueLyser LT?

Any number of samples between 1 and 12 can be processed on the TissueLyser LT. If processing either 1 sample or 11 samples, load an additional, empty sample tube to balance the TissueLyser LT Adapter. For more information please see the TissueLyser LT Handbook.

 

See: TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT works.

FAQ ID -2547
What are the dimensions of the TissueLyser LT?

The TissueLyser LT has the following weight and dimensions:

  • Weight: Approx. 7.35 kg (net); approx. 9 kg (gross)
  • Width: 150 mm
  • Depth: 270 mm
  • Height: 280 mm
FAQ ID -2545
What is the typical amount of tissue used for disruption with the TissueLyser LT?
The amount of starting material is dependent on the sample material that should be disrupted. For plant tissues up to 100 mg, and for human or animal tissues 25 to 30 mg, can be disrupted with the TissueLyser LT. When disrupting yeast up to 5 x 10^7 cells, and when disrupting bacteria up to 7.5 x 10^8 cells, can be used. Using more than the recommended amount of starting material will significantly decrease disruption efficiency.
FAQ ID -2552
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?

Yes, if using fresh samples that are not stabilized with a stabilization reagent (e.g., RNAprotect Tissue Reagent or Allprotect Tissue Reagent) the TissueLyser LT Adapter can be precooled on dry ice. During the disruption process the adapter will passively cool the samples to prevent heating and degradation of nucleic acids or proteins.

See:  TissueLyser Virtual Demo for a virtual demonstration of this precooling process with the TissueLyser LT.


FAQ ID -2548
Can the TissueLyser LT Adapter be removed for cleaning?

Yes, the TissueLyser LT Adapter can completely be removed from the instrument for cleaning. For more information on how to clean or decontaminate the instrument and the adapter please refer to chapter 6.1 in the TissueLyser LT User Manual on regular maintenance. The TissueLyser LT Adapter must not be autoclaved.

 

See:  TissueLyser Virtual Demo for a virtual demonstration on how the TissueLyser LT Adapter is used 

FAQ ID -2546
What kind of sample tubes are recommended to use with the TissueLyser LT?
The TissueLyser LT has been thoroughly tested with the Sample Tubes RB (2 ml) catalog number 990381. We do not recommend the use of any other tubes with the instrument.
FAQ ID -2553
What size bead should I use for tougher samples with the TissueLyser LT?

In general, disruption works very well using one stainless steel bead with a diameter of 5 mm. However, for tougher samples we recommend the use of two 7mm stainless steel beads to optimize the effectiveness of disruption.

 

See:  TissueLyser Virtual Demo for a demonstration on how these beads are used with the TissueLyser LT.

FAQ ID -2554