CompactPrep Plasmid Kits

For fast purification of up to 750 µg molecular biology grade plasmid or cosmid DNAFor fast purification of up to 750 µg molecular biology grade plasmid or cosmid DNA

Features

  • Purification of up to 24 samples in <60 minutes
  • Low elution volumes for highly concentrated DNA
  • Bench-top processing with no need for large centrifuges
  • LyseBlue for optimum lysis and maximum DNA yield
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CompactPrep Plasmid Midi Kit (25)

Cat. No. / ID: 12843

25 CompactPrep Midi Columns, Extender tubes, Reagents, Buffers, LyseBlue
Column type
Midi
Maxi
CompactPrep Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

CompactPrep Plasmid Kits provide fast spin column-based large-scale plasmid purification. Up to 24 samples can be processed in parallel in less than 60 minutes using a vacuum manifold (e.g., QIAvac 24 Plus). Handling of the samples is fast and easy. CompactPrep Plasmid Kits require use of a vacuum device (e.g., QIAvac 24 Plus). Note: CompactPrep Plasmid Kits do not contain QIAfilter Cartridges and require centrifugation for lysate clearing.

For optimal results it is recommended to use this product together with QIAvac 24 Plus.

Performance

CompactPrep Plasmid Kits enable fast, large-scale plasmid purification. The use of a vacuum manifold allows up to 24 samples to be purified in parallel on a single workbench (see figure  "Microcentrifuge column format") for plasmid midi and plasmid maxi preps. Up to 200 µg plasmid DNA is purified in less than 60 minutes from 25 ml (high-copy plasmids) or 50 ml (low-copy plasmids) LB medium using the CompactPrep Plasmid Midi Kit. DNA is eluted in 100 µl and concentrations are typically 1–2 µg/µl. The CompactPrep Plasmid Maxi Kit enables purification of up to 750 µg plasmid DNA in less than 60 minutes from 100 ml (high-copy plasmid) or 250 ml (low-copy plasmid) LB medium. DNA is eluted in 200 µl and concentrations are typically 3–4 µg/µl. CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA with low endotoxin levels (see figure  "Low endotoxin levels").

Maximum plasmid yields using LyseBlue


LyseBlue reagent is now also included in CompactPrep Kits ensuring high DNA yields. Using a simple visual identification system, LyseBlue reagent prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, cell debris and genomic DNA. LyseBlue is optional and provided in a separate vial within the kit.

See figures

Principle

CompactPrep Plasmid Kits provide a novel method for fast large-scale plasmid preparation. The procedure is based on a nonchaotropic binding chemistry, reducing the total preparation volume to miniprep scale. All protocol steps after cell harvest can be performed at the bench using QIAfilter cartridges, a vacuum manifold and a standard microcentrifuge. The unique kit chemistry and design of the CompactPrep column ensure large-scale plasmid prep yields using a spin column format.

Procedure

CompactPrep Plasmid Kits provide fast, large-scale, nonchaotropic silica-based plasmid DNA preparation. DNA binding and washing, using a spin column on a vacuum manifold (e.g., QIAvac 24 Plus), and DNA elution, using a microcentrifuge, enables the entire plasmid purification process to take place at the bench.

Applications

CompactPrep Plasmid Kits provide molecular biology grade plasmid DNA suitable for use in routine applications, including:

  • High-quality automated sequencing
  • Restriction digestion
  • Ligation and transformation
  • In vitro transcription and translation
  • Transfection of robust cells

Comparison of CompactPrep Plasmid Kits

Features CompactPrep Plasmid Midi Kit CompactPrep Plasmid Maxi Kit
Applications Automated sequencing, restriction digestion, transfection Automated sequencing, restriction digestion, transfection
Culture volume/starting material 25–100 ml culture volume 50–250 ml culture volume
Plasmid type Plasmids, cosmids Plasmids, cosmids
Processing Manual (centrifugation and vacuum) Manual (centrifugation and vacuum)
Samples per run (throughput) 1–24 samples per run 1–24 samples per run
Technology Silica technology Silica technology
Time per run or per prep <20 minutes <20 minutes
Yield <200 µg <750 µg

CompactPrep Plasmid Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Supporting data and figures

Resources

Quick-Start Protocols (1)

FAQ

Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?

The quality of plasmid DNA prepared with CompactPrep Plasmid Midi and Maxi Kits is already very good – both Kits are suited for all routine applications including transfections of robust cell lines.

Due to a new wash-buffer included in the CompactPrep Plasmid Mega and Giga Kits, these kits yield plasmid DNA of even better quality, suitable for transfection of more sensitive cell lines as well.

 

FAQ ID -1882