QIAGEN Genomic-tips

For isolation of up to 20 µg, 100 µg or 500 µg high-molecular-weight DNA from a wide range of samples

Features

  • Minimal mechanical shearing allows isolation of high-molecular-weight DNA up to 150 kb in size
  • No phenol or chloroform extractions
  • Convenient, parallel processing of multiple samples
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QIAGEN Genomic-tip 20/G

Cat. No. / ID: 10223

25 columns
Column type
20/G
100/G
500/G
QIAGEN Genomic-tips are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

QIAGEN Genomic-tips are gravity-flow, anion-exchange tips that allow efficient purification of genomic DNA from a wide range of biological samples. The purified DNA is sized up to 150 kb with an average size of 50–100 kb

Performance

The QIAGEN Genomic-tip procedure is very gentle and results in negligible DNA shearing. DNA purified with QIAGEN Genomic-tips is sized up to 150 kb with an average length of 50–100 kb (see figure " Genomic DNA of up to 150 kb"). The DNA is free of all contaminants such as RNA, protein and metabolites and has A260 / A280 ratios between 1.7 and 1.9.


QIAGEN Genomic-tips come in a range of sizes to suit every preparation need and are supplied with a comprehensive handbook containing detailed protocols for genomic DNA preparation from each sample type. Specific lysis buffers for blood, tissue, cultured cells, yeast and bacteria and all other necessary buffers are provided in the Genomic DNA Buffer Set which can be purchased separately.


Purified DNA has been used in direct bacterial-genome sequencing (see figure " Direct sequencing of bacterial genomic DNA").

QIAGEN Genomic-tip specifications

Sample source Amount Yield (µg)
  Genomic-tip 20/G Genomic-tip 100/G Genomic-tip 500/G Genomic-tip 20/G Genomic-tip 100/G Genomic-tip 500/G
Whole blood (human) 1 ml 5 ml 20 ml 15–20 80–100 350–400
Cultured cells (HeLa) 5 x 106 2 x 107 1 x 108 15–20 80–100 350–450
Tissue (liver) 15 mg 80 mg 350 mg 15–20 80–100 350–450
Yeast (S. cerevisiae) 1 ml 5 ml 20 ml 18–20 85–95 350–450
Gram– bacteria (E. coli) 0.6–1.2 ml 3–6 ml 15–30 ml 16–20 85–95 300–400
Gram+ bacteria (B. subtilis) 1.2–1.8 ml 6–9 ml 30–45 ml 16–20 85–95 300–400
See figures

Principle

QIAGEN Genomic-tips use unique QIAGEN anion-exchange technology for high-molecular-weight DNA isolation from a wide range of biological samples without phenol or chloroform. Lysis buffers are optimized for different sample types and provide immediate denaturation of proteins such as nucleases, histones and DNA-binding proteins, as well as potentially infectious viral particles. Under the pH and low-salt conditions provided by the buffer, DNA binds to the QIAGEN Resin in the column. At the same time, other cell constituents such as proteins, carbohydrates and metabolites flow through. Purified DNA is eluted in high-salt buffer. Genomic-tips operate by gravity flow, and can be left unattended without running dry. This reduces hands-on time to a minimum and makes the procedure ideal for simultaneous processing of multiple samples.

Procedure

Samples are first lysed (tissue samples are mechanically disrupted) and proteins simultaneously denatured in the appropriate lysis buffer (see flowchart " QIAGEN Genomic-tip procedure"). QIAGEN Protease or Proteinase K is then added and after a suitable incubation period, lysates are loaded onto the QIAGEN Genomic-tip. DNA binds to the column while other cell constituents pass through. Following a wash step to remove any remaining contaminants, pure, high-molecular-weight DNA is eluted and precipitated with isopropanol. Hands-on time for the complete procedure is just 20 minutes for bacteria, 30 minutes for blood and cultured cells and 40 minutes for tissue and yeast.

See figures

Applications

DNA purified with QIAGEN Genomic-tips is well suited for use in the following applications:

  • RFLP analysis
  • Analysis of gene targeting
  • Screening of transgenic animals
  • DNA fingerprinting studies
  • Southern blotting

Supporting data and figures

Resources

User-Developed Protocols (7)
Supplementary Protocols (1)
This protocol is designed for isolation of up to 200 μg RNA from 150 mg plant tissue or up to 1 mg RNA from 600 mg plant tissue and is for use with QIAGEN-tip 100 or QIAGEN-tip 500, respectively.
Kit Handbooks (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.

Publications

Similarity and differences in the Lactobacillus acidophilus group identified by polyphasic analysis and comparative genomics.
Berger B; Pridmore RD; Barretto C; Delmas-Julien F; Schreiber K; Arigoni F; Brüssow H;
J Bacteriol; 2006; 189 (4):1311-21 2006 Dec 1 PMID:17142402
A genome-wide map of diversity in Plasmodium falciparum.
Volkman SK; Sabeti PC; DeCaprio D; Neafsey DE; Schaffner SF; Milner DA Jr; Daily JP; Sarr O; Ndiaye D; Ndir O; Mboup S; Duraisingh MT; Lukens A; Derr A; Stange-Thomann N; Waggoner S; Onofrio R; Ziaugra L; Mauceli E; Gnerre S; Jaffe DB; Zainoun J; Wiegand RC; Birren BW; Hartl DL; Galagan JE; Lander ES; Wirth DF;
Nat Genet; 2006; 39 (1):113-9 2006 Dec 10 PMID:17159979
Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection.
Kamachi K; Toyoizumi-Ajisaka H; Toda K; Soeung SC; Sarath S; Nareth Y; Horiuchi Y; Kojima K; Takahashi M; Arakawa Y;
J Clin Microbiol; 2006; 44 (5):1899-902 2006 May PMID:16672435
Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase.
Qin J; Rosen BP; Zhang Y; Wang G; Franke S; Rensing C;
Proc Natl Acad Sci U S A; 2006; 103 (7):2075-80 2006 Feb 1 PMID:16452170
Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes.
Dixon LA; Murray CM; Archer EJ; Dobbins AE; Koumi P; Gill P;
Forensic Sci Int; 2005; 154 (1):62-77 2005 Jan 20 PMID:16182950

FAQ

Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?

 Yes, please follow the User-Developed Protocol 'Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip' (QG08).

FAQ ID -905
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?

Yes, please follow the User-Developed Protocol 'Isolation of genomic DNA from flies using the QIAGEN Genomic-tip 100/G' (QG05).

FAQ ID -903
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
Do you have a protocol for isolation of genomic DNA from insects?

Yes, we have the following protocols:

  • Isolation of genomic DNA from mosquitoes or other insects using the QIAGEN Genomic tip (QG06).
  • Purification of total DNA from insects using the DNeasy Blood & Tissue Kit (DY14).
FAQ ID -904
Do you have a protocol for the purification of DNA from fruit flies?

Using Gentra Puregene Cell kit:

 

• Purification of archive-quality DNA from up to 30 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG20)

• Purification of archive-quality DNA from 100 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG21)

• Purification of archive-quality DNA from 300–700 Drosophila melanogaster using the Gentra Puregene Cell Kit (PG22)

 

Using the QIAGEN Genomic tips:

• Isolation of genomic DNA from flies using the QIAGEN Genomic-tip 100/G (QG05)

FAQ ID -1970
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?

Yes, please follow the User-Developed Protocol 'Isolation of genomic and plasmid DNA from cell cultures using the QIAGEN Genomic-tip' (QG04).

FAQ ID -902
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

Yes, we have the following protocols:

  • Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

  • Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
  • Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
  • Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).
FAQ ID -900. Test
Do you have a protocol for the isolation of genomic DNA from fungi?

Yes, we have the following protocols:

  • Isolation of genomic DNA from fungi (culture and blood) using the QIAamp DNA Mini Kit (QA09).
  • Isolation of genomic DNA from plants and filamentous fungi using the QIAGEN Genomic-tip (QG08).
FAQ ID -911
Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?

Yes, please follow the User-Developed Protocol 'Isolation of genomic and viral DNA from lymphocytes using the QIAGEN Genomic-tip' (QG03). The protocol is for use with the QIAGEN Genomic-tip 20/G.

FAQ ID -901
What do you recommend for the cleanup of genomic DNA (gDNA)?

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

 

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

 

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618
What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
Genomic DNA purified using QIAGEN Genomic-tips ranges in size from 20–150 kb, with an average length of 50–100 kb. Vortexing the lysate for about 20 seconds may reduce the size of the genomic DNA slightly to 20–130 kb, but can help to improve flow rates.
FAQ ID -142