BioSprint DNA Blood Kits

For rapid and economical DNA purification from a wide range of sample types

Features

  • High-throughput isolation of DNA
  • Maximal flexibility to accommodate challenging sample material
  • Complete removal of contamination and inhibitors
  • No organic extraction or alcohol precipitation
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BioSprint 96 DNA Blood Kit (48)

Cat. No. / ID: 940054

For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
Preparations
48
384
BioSprint DNA Blood Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

BioSprint DNA Blood Kits provide all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots and buccal swabs using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.

Performance

DNA can be successfully purified from a variety of sample types (see table “Typical yields from a range of sample types”).

Typical yields from a range of sample types

Sample type Amount of sample DNA (µg)
    BioSprint 15 BioSprint 96
Muscle, bovine 25 mg 16.2 ± 2.5 13.5 ± 1.5
Heart, bovine 25 mg 5.9 ± 2.6 3.4 ± 0.6
Spleen, bovine 25 mg 69.1 ± 23.6 59.1 ± 4.8
Lung, bovine 25 mg 13.8 ± 7.2 14.7 ± 5.5
Liver, bovine 25 mg 77.8 ± 29.4 74.0 ± 22.3
Kidney, bovine 25 mg 26.2 ± 18.8 33.5 ± 5.4
Ear, sheep 30 mg 20.31 ± 1.8
Tail, mouse 1.2 cm (~25 mg) 32.7 ± 4.6 30.9 ± 4.5
HL-60 cultured cells 2 x 106 cells 10.1 ± 4.7 9.6 ± 5.6

Blood, human

(5–7 x 106 cells per ml)

200 µl 4.5–9.0 4.5–9.0
Blood, bovine 200 µl 6.2–8.0 4.4–7.0
Blood, sheep 200 µl 5.6–11.2 1.2–8.0
Blood, pig 200 µl 4.5–9.0 4.5–9.0
Blood, dog 200 µl 6.7–12.3 6.9–16.1
Blood, cat 100 µl 4.4–8.3 2.9–6.3
Blood, mouse 100 µl 2.0–8.0 1.5–3.0
Blood, rat 100 µl 1.0–4.0 1.0–3.0
Blood, bird 10 µl* 15.0 ±6.3
Blood, fish 5 µl* 7.1–10.0
Dried blood spots, 903 Specimen Collection Paper 1 punch (6 mm [1/4 inch] diameter) 0.30 0.10
Dried blood spots, FTA Card 1 punch (6 mm [1/4 inch] diameter) 0.12 0.05
Buccal swabs 1 swab 0.8–2.0 0.8–2.0

Purified DNA is up to 50 kb in size (see figures " Purification of high-quality genomic DNA from mouse tail" and " Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure " High PCR performance of purified DNA").

See figures

Principle

BioSprint DNA Blood Kits are used with the BioSprint 15 or BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from cells, tissue and blood or blood-related products. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.

See figures

Procedure

Genomic DNA can be purified from cells, tissue and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure " BioSprint DNA procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.

For purification of genomic DNA from tissue, dried blood spots or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with BioSprint DNA Blood Kits). For processing in 96-well format, we recommend sealing S-Blocks with Tape Pads during sample processing (not needed for rapid blood protocol).

See figures

Applications

BioSprint DNA Blood Kits purify DNA for use in a range of downstream applications, including:

  • Southern blotting
  • PCR and real-time PCR
  • Genotyping

Comparison of BioSprint DNA Blood Kits

Features BioSprint 15 DNA Blood Kit BioSprint 96 DNA Blood Kit
Applications PCR, real-time PCR, genotyping, blotting PCR, real-time PCR, genotyping, blotting
Elution volume 200 µl Custom
For automated processing BioSprint 15 workstation BioSprint 96 workstation
Format 5-tube strip, S-Block S-Block, 96-well plate
Main sample type Blood, tissue, cells Blood, tissue, cells
Processing Automated Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA DNA
Sample amount 100 µl/25 mg/2 x 10e6 100–250 µl
Technology Magnetic particles Magnetic particles
Time per run or per prep 45 minutes 45 minutes
Yield Varies Varies

Supporting data and figures

Resources

Brochures & Guides (1)
Rapid and economical preparation of 1–15 or 1–96 samples using magnetic-particle technology
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (1)
For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 96 workstation
Quick-Start Protocols (1)

FAQ

Can the BioSprint instruments be placed in a cool room?

No, the BioSprint Instruments should not be operated in a cool room. Optimal operation temperature for BioSprint is 10-40°C. Operation at 4°C may harm the instruments.

 

FAQ ID -938
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?

Yes, please refer to the protocols mentioned in the following handbooks:

FAQ ID -983
Do you have stability data for genomic DNA isolated with the BioSprint Kits?

Yes. We have shown that DNA purified with the BioSprint DNA Kits (e.g., the BioSprint 15 DNA Blood Kit, BioSprint 15 DNA Plant Kit, BioSprint 96 DNA Blood Kit and BioSprint 96 DNA Plant Kit) is stable for at least 2 years when being stored at either 2-8°C or -20°C. Our data indicate however that DNA stability is dependent upon storage conditions. For additional information see also FAQ 518.

 

FAQ ID -1318
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
How do I perform a DNA precipitation to concentrate my sample?
  • Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
  • Mix, and store at -20°C for at least 1 hour to precipitate the DNA
  • Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15-20 minutes
  • Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
  • Allow the DNA pellet to air-dry
  • resuspend the DNA in a suitable volume of sterile TE buffer or distilled water
FAQ ID -305
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?

The BioSprint 96 instrument has a magnetic-particle collecting efficiency above 95%. However, to avoid particle carryover from the eluate plate to subsequent applications, you can use a plate magnet while pipetting.

 

FAQ ID -2073
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?

High-quality genomic DNA can be purified from up to 96 pre-lysed samples in just 20 minutes. Including setup, the total time per run for the blood and buffy coat protocol is 45 minutes. For the other protocols (e.g. tissue), the run time will be slightly higher depending on the time of pre-treatment.

 

FAQ ID -622
How to quantitate DNA purified using the BioSprint 96?

Measure the absorbance of the eluate obtained on the BioSprint 96 instruments at 260 nm, 280 nm and 320 nm. Carryover of magnetic particles in the eluates may affect the A260 and A280 readings, but should not affect the performance of the DNA in downstream applications. Subtract the absorbance reading obtained at 320 nm from the readings obtained at 260 nm and 280 nm to correct for the presence of residual magnetic particles.

FAQ ID -773
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
Are the BioSprint 15/96 instruments stand-alone workstations?

Yes, the BioSprint 15/96 instruments are stand-alone instruments and do not need to be connected to a computer for the actual run. However, a computer is needed to import and export protocols.

FAQ ID -620