AllPrep DNA/mRNA Nano Kit

For the simultaneous purification of genomic DNA (gDNA) and messenger RNA (mRNA) from low-biomass samples

Features

  • Simultaneous gDNA and mRNA isolation
  • Maximal yields from minimal cell quantities
  • High-molecular-weight, sequencing-ready gDNA
  • Messenger RNA suitable for single-cell RNA-seq application
  • Magnetic-bead-based system
AllPrep DNA/mRNA Nano (12)

Cat. No. / ID: 80272

For 12 preps: Oligo-dT magnetic beads, DNA-isolation beads, collection tubes, RNase-free water and buffers
KitAccessories
AllPrep DNA/mRNA Nano
Accessories
The AllPrep DNA/mRNA Nano Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Collect genetic and transcriptomic information from CTCs and rare cells, with minimal cell quantities, by isolating gDNA and mRNA from the same sample, at the same time.

The AllPrep DNA/mRNA Nano Kit uses a two-step magnetic-bead-based method that lets you maximize mRNA capture and isolate high-molecular-weight DNA for multiomics studies.

Performance

The AllPrep DNA/mRNA Nano Kit purifies high-quality gDNA and mRNA from the same cell (see figure “ Simultaneous mRNA and gDNA purification”) or low-biomass input samples (see figures “ Messenger RNA for single-cell RNA-seq applications”, “ Sequencing-ready gDNA from low cell numbers”, and “ Minimal amount of cells required”).

The kit’s simple and convenient procedure does not require the use of toxic substances such as phenol (see figure “ Magnetic-bead-based system”).

Genomic DNA purified with the AllPrep mRNA/DNA Nano Kit procedure has an average length of 60–100 kb, depending on homogenization conditions (see figure “ High-molecular-weight gDNA”). Compared to products from other suppliers, the AllPrep mRNA/DNA Nano Kit provides nucleic acids of significantly higher yields (see figure “ Maximal DNA and mRNA yields”).

See figures

Principle

Unlike other procedures where the biological sample or purified total nucleic acids are divided into two before being processed separately, the AllPrep DNA/mRNA Nano Kit purifies gDNA and mRNA simultaneously from the same precious sample. It also allows the parallel processing of multiple samples in less than 75 minutes.

The kit is compatible with small amounts of low-biomass samples such as CTCs, rare cells and low numbers of cells of animal or human origin.

The use of toxic substances such as phenol and/or chloroform, and tedious methods such as alcohol precipitation, are replaced by safe and simple magnetic-bead-based workflows using a magnetic rack such as the AdnaMag-S.

Procedure

The AllPrep mRNA/DNA Nano Kit’s  magnetic-bead-based system purifies mRNA using oligo-dT magnetic beads, while gDNA is purified using DNA-affinity beads.

See figures

Applications

Genomic DNA purified with the AllPrep DNA/mRNA procedure has a high molecular weight of up to 100 kb. This purified DNA is suitable for any downstream application, such as PCR, multiplex PCR, dPCR, pyrosequencing or NGS.

The purified mRNA is of high quality and can be used in any application, such as RT-PCR, real-time RT-PCR, or cDNA generation, including the AdnaTest procedure for molecular characterization of CTCs.

Supporting data and figures

Specifications

FeaturesSpecifications
applicationsTranscriptome and genome profiling, real-time PCR, cDNA synthesis, NGS, RNA seq
elutionvolume25 µl
formatMagnetic-bead-based system
mainsampletypeLow-biomass samples (rare cells, CTCs, fetal cells, stem cells, T-cells)
processingManual (magnetic rack)
productusePurification of full-length messenger RNA and high-molecular-weight genomic DNA
sampleamountDown to 1 cell
technologyMagnetic beads
timeperrunorperprep75 min per prep

Resources

Kit Handbooks (1)
Quick-Start Protocols (1)
Brochures & Guides (1)

FAQ

What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?
For PCR applications using RNA, the minimum amount is 1 cell; for Pyrosequencing, 5 cells; for NGS applications, 20 cells.
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Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?
No, the AllPrep DNA/mRNA Nano Kit isolates messenger RNA only. For the isolation of microRNA we recommend to use the miRNeasy Micro Kit.
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