EZ1 DNA Kits

For automated purification of high-quality DNA from 1–6 or 1–14 samples per run

Features

  • Rapid purification of up to 9.5 µg DNA from whole blood
  • Purification of up to 14 µg DNA from buffy coat
  • Processing of 200–350 µl whole blood samples
  • Standardized processing of up to 40 mg tissue samples
EZ1 DNA Blood 200 µl Kit (48)

Cat. No. / ID: 951034

For 48 preps: Reagent Cartridge (Blood 200 µl), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml)
CA$487.00
Sample type
Blood
Tissue
Sample volume
200 µl
350 µl
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EZ1 DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

EZ1 DNA Kits contain all required reagents and labware for rapid, automated purification of DNA from up to 200 μl blood samples, up to 350 μl blood or buffy coat samples or up to 40 mg tissue samples using magnetic-particle technology. The kits can be used with the EZ1 Advanced XL (1–14 samples per run), EZ1 Advanced (1–6 samples per run) and BioRobot EZ1 (1–6 samples per run) instruments. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument.

These EZ1 Kits can also be automated with EZ2 Connect, which features cardless protocols, expanded applications and remote connectivity options. Click here to learn more about the EZ2 Connect, or contact your sales representative to discover how easily you can get an EZ2 Connect for your lab.

Performance

The automated procedure provides high repeatability (see figures  "High-quality DNA from whole blood" and  "Reproducible yields of high-quality DNA from whole blood") and linear yields over a range of starting amounts. DNA purified using EZ1 DNA Kits gives high-performance results in downstream applications, such as PCR (see figures  "High-quality DNA for sensitive and specific analyses" and  “Consistent PCR results”).

See figures

Principle

Automated purification with EZ1 DNA Kits requires use of EZ1 instruments and EZ1 DNA Cards (see table “EZ1 DNA Kits and EZ1 DNA Cards”). EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure. Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–14 samples (EZ1 Advanced XL) or 1–6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination.

EZ1 DNA Kits and EZ1 DNA Cards

EZ1 DNA Kit EZ1 DNA Card
EZ1 DNA Blood 200 µl Kit EZ1 Advanced XL DNA Blood Card
EZ1 Advanced DNA Blood Card
EZ1 DNA Blood Card
EZ1 DNA Blood 350 µl Kit EZ1 Advanced XL DNA Blood Card
EZ1 Advanced XL DNA Buffy Coat Card
EZ1 Advanced DNA Blood Card
EZ1 Advanced DNA Buffy Coat Card
EZ1 DNA Blood Card
EZ1 DNA Buffy Coat Card
EZ1 DNA Tissue Kit EZ1 Advanced XL DNA Tissue Card
EZ1 Advanced DNA Tissue Card
EZ1 DNA Tissue Card

Procedure

Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. Whole blood samples (200 µl or 350 µl) and pretreated tissues are loaded onto the instrument. Genomic DNA is then purified in one step following the automated EZ1 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

The high-quality DNA obtained using EZ1 DNA Kits is suited for use in many applications, such as:

  • Population genetics and pharmacogenomic research
  • PCR and real-time PCR
  • Sequencing
  • Oncology research
  • Genetic identity testing and forensics
  • Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies

EZ1 DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Comparison of EZ1 DNA Kits

Features EZ1 DNA Blood 200 µl Kit EZ1 DNA Blood 350 µl Kit EZ1 DNA Tissue Kit
Applications PCR, real-time RT-PCR, genotyping PCR, real-time RT-PCR, genotyping PCR, real-time RT-PCR, genotyping
Elution volume 200 µl 200 µl 200 µl
For automated processing EZ1 Advanced XL, EZ1 Advanced, BioRobot EZ1 EZ1 Advanced XL, EZ1 Advanced, BioRobot EZ1 EZ1 Advanced XL, EZ1 Advanced, BioRobot EZ1
Format Sample tube Sample tube Sample tube
Main sample type Whole blood Whole blood Tissue samples
Processing Automated Automated Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA DNA DNA
Sample amount 200 µl 350 µl 10–40 mg
Technology Magnetic particles Magnetic particles Magnetic particles
Time per run or per prep 15–20 minutes 15–20 minutes 40 minutes
Yield 2.6–6 µg 5–12 µg 9–28 µg

Supporting data and figures

Resources

Kit Handbooks (2)
EZ1 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments
EZ1 DNA Blood 200 μl Kit
EZ1 DNA Blood 350 μl Kit
For automated purification of DNA from blood and buffy coat using EZ1 instruments
Brochures & Guides (1)
Fully automated low- to medium-throughput purification of nucleic acids

FAQ

Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
Is training of lab personnel included with the purchase of a BioRobot EZ1?

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

  • Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
  • Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
  • Collect purified nucleic acids when the protocol run ends

 

FAQ ID -1239
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?

Yes, please follow the Supplementary Protocol 'Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit' (MA15).

FAQ ID -990
How should I prepare buffy coat samples for use on the BioRobot EZ1?

Making consistent buffy samples takes practice. Serum and erythrocytes are easily isolated alongside the 10x concentrated leukocyte fraction. Our Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02) contains instructions and recommendations for preparation of buffy coat for DNA isolation on the BioRobot EZ1.

FAQ ID -1249
How can we get our BioRobot EZ1 upgraded when new protocols are launched?

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

 

FAQ ID -1241
Where can I find information about the worktable setup on the BioRobot EZ1?

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

 

FAQ ID -1243
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?

Yes, please check the EZ1 DNA Tissue Handbook for instructions on pretreatment and extraction protocol.

 

FAQ ID -1260
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?

For storage of DNA purified from forensic samples using the EZ1 DNA Tissue Kit in a buffered solution such as TE rather than water (used for elution in the EZ1 forensic protocols) we suggest the following:

Before storing the DNA, add 1/10 volume of 10x Forensic TE buffer (100 mM Tris·Cl, 1 mM EDTA, pH 8.3) to each sample. For example, add 5 µl of 10x Forensic TE buffer if you eluted in 50 µl. The DNA is ready to use, or can be stored at 2–8°C for 24 h, or at –20°C for longer periods.

FAQ ID -734
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
Yes, please follow the Supplementary Protocol 'Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 µl Kit' (MA02).
FAQ ID -985
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

 

FAQ ID -1240
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12
What disposables are required for nucleic acid isolations on the BioRobot EZ1?

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

 

FAQ ID -1246