artus WNV LC RT-PCR Kit RUO

The artus WNV LC PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation.

To ensure sensitivity, the artus WNV LC RT-PCR Kit has been optimized to detect low numbers of West Nile virus RNA. The analytical sensitivity of the artus WNV LC RT-PCR Kit is 2.4 copies/µl in the PCR on the LightCycler 1.1/1.2/1.5 Instruments.

The artus WNV LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the West Nile virus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus WNV LC RT-PCR Kit provides 4 quantitation standards (see figure " Reliable quantitation of WNV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical WNV RT-PCR is not reduced.

The artus WNV LC RT-PCR Kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

The artus WNV LC RT-PCR Kit enables rapid and sensitive detection of West Nile virus RNA from serum, plasma, and cerebral spinal fluid (CSF) with accurate quantification. The kit is available for use on the LightCycler 1.1/1.2/1.5 Instruments.