QIAsymphony DSP Virus/Pathogen Kit

For automated purification of viral nucleic acids or bacterial/pathogen DNA from 1–96 samples

Features

  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents
  • Optimized protocols provide high yields of pure nucleic acids
  • Novel, prefilled reagent cartridges for ease of use

Products

The QIAsymphony DSP Virus/Pathogen Kits are intended for in-vitro diagnostic use.
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QIAsymphony DSP Virus/Pathogen Midi Kit

Cat. No. / ID: 937055

For 96 preps (1000 µl each): includes 2 reagent cartridges and enzyme racks and accessories
CA$920.00
Add to cart
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QIAsymphony DSP Virus/Pathogen Mini Kit

Cat. No. / ID: 937036

For 192 preps (200 µl each): includes 2 reagent cartridges and enzyme racks and accessories
CA$1,185.00
Add to cart

Product Details

QIAsymphony DSP Virus/Pathogen Kits, in combination with the QIAsymphony SP, enable automated purification of viral nucleic acids and bacterial/pathogen DNA from a broad range of sample materials for in vitro diagnostic use. Kits are available in mini and midi format for sample volumes of 200 μl and up to 1000 μl. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Principle

QIAsymphony DSP Virus/Pathogen Kits can be used to purify viral nucleic acids from serum, plasma, or CSF, and viral nucleic acids and bacterial DNA can be purified from respiratory samples such as swabs, aspirates, sputum, broncheolar lavage (BAL), as well as urine and urogenital swabs (cervical and urethral). The kits can be used to purify nucleic acids from a broad range of DNA and RNA viruses, as well as bacterial DNA from Gram-negative and Gram-positive bacteria.

QIAsymphony DSP Virus/Pathogen Kits combine the speed and efficiency of silica-based purification of viral and bacterial nucleic acids with the convenient handling of magnetic particles (see " Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony DSP Virus/Pathogen Kits are ready for direct use in downstream applications (e.g., PCR). The user can choose between different elution volumes.

See figures

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see " QIAsymphony DSP Virus/Pathogen procedure"). Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Protocols for use with the QIAsymphony DSP Virus/Pathogen Kit
ProtocolKit Sample material Volume processed
Cellfree 200 QIAsymphony DSP Virus/Pathogen Mini Kit Plasma, serum, and CSF 200 µl
Cellfree 500 QIAsymphony DSP Virus/Pathogen Midi Kit Plasma, serum, and CSF 500 µl
Cellfree 1000 QIAsymphony DSP Virus/Pathogen Midi Kit Plasma, serum, and CSF 1000 µl
Complex 200 QIAsymphony DSP Virus/Pathogen Mini Kit Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum) and urogenital samples (urine, transport media) 200 µl
Complex 400 QIAsymphony DSP Virus/Pathogen Midi Kit Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum) and urogenital samples (urine, transport media) 400 µl
Complex 800 QIAsymphony DSP Virus/Pathogen Midi Kit Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum) and urogenital samples (urine, transport media) 800 µl

See figures

Applications

QIAsymphony DSP Virus/Pathogen Kits enable sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.

Supporting data and figures

Resources

Application/Protocol Documents (9)
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
For use with software version 4.0 or higher
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Labware Documents (1)
For use with software version 5.0 or higher

FAQ

What sample input formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
Tube carriers for up to 24 tubes (primary or secondary tubes or tubes containing internal controls) can be used. A list of compatible labware formats for sample input can be found at www.qiagen.com/goto/dsphandbooks. Slots 1–4 are used for samples to be processed. The internal control-carrier RNA-Buffer AVE mixture is loaded into Slot 5.
FAQ ID -2936
Why do I have to use an ACS (Assay Control Set)?
The ACS specifies the combination of a protocol and a predefined carrier RNA–internal control mixture. The user can define an internal control mixture using the QIAsymphony Management Console. A predefined internal control-carrier RNA-Buffer AVE mixture can be automatically identified by the instrument using a bar code label or it can be chosen manually.
FAQ ID -2933
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927
What elution formats can be used for the QIAsymphony DSP Virus/Pathogen protocols?
We recommend using racked Elution Microtubes CL (cat. no. 19588) for elution. Alternatively, 2 ml Sarstedt tubes (with screw caps, Sarstedt cat. no. 72.693) can be used. A list of compatible labware formats for eluates can be found at www.qiagen.com/goto/dsphandbooks.
FAQ ID -2937
Which kit size should be used for each QIAsymphony DSP Virus/Pathogen protocol?

The QIAsymphony DSP Virus/Pathogen Mini Kit is used with the Cellfree200, Complex200 and Complex200_OBL protocols.

The QIAsymphony DSP Virus/Pathogen Midi Kit is used with the Cellfree500, Cellfree1000, Complex400, Complex400_OBL, Complex800, and Complex800_OBL protocols.

All complex protocols require Buffer ATL, which is not included in the QIAsymphony DSP Virus/Pathogen Kits. Buffer ATL can be ordered separately (Buffer ATL (4 x 50 ml), cat. no. 939016).

FAQ ID -2930
Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
Yes. The QIAsymphony DSP Virus/Pathogen Kits are designated “For In Vitro Diagnostic Use”. It is the user’s responsibility to validate system performance for any procedures used in their laboratory that are not covered by the QIAGEN performance evaluation studies. The system performance has been established in performance evaluation studies purifying viral DNA and RNA from human serum, plasma, or cerebrospinal fluid (CSF), and purifying viral DNA and RNA, as well as bacterial DNA, from respiratory and urogenital samples. To minimize the risk of a negative impact on the diagnostic results, adequate controls for downstream applications should be used. For further validation, the guidelines of the International Conference on Harmonisation of Technical Requirements (ICH) in ICH Q2 R(1) Validation of Analytical Procedures: Text and Methodology are recommended. Any diagnostic results that are generated must be interpreted in conjunction with other clinical or laboratory findings.
FAQ ID -2939
What sample types can be processed using the QIAsymphony DSP Virus/Pathogen protocols?
Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.
FAQ ID -2928
Which elution volumes can be used for the QIAsymphony DSP Virus/Pathogen protocols?
For all cellfree and complex protocols, an eluate volume of 60 µl, 85 µl, or 110 µl can be selected. These are the minimum accessible volumes of eluate in the final elution tube. The initial elution volumes, which have to be used for the internal control, as well as titer calculations, are 90 µl, 115 µl, and 140 µl.
FAQ ID -2938
Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
Pretreatment can improve the nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Performance may be improved for Gram positive bacteria by pretreatment with lysozyme. Pretreatment protocols are provided in the QIAsymphony DSP Virus/Pathogen Handbook.
FAQ ID -2931
How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
The QIAsymphony DSP Virus/Pathogen Mini kit can be used to process 192 samples (96 per reagent cartridge). The Midi kit can be used to process 96 samples (48 per reagent cartridge) using the Cellfree1000, Complex800, and the Complex800_OBL protocols. The Midi Kit can also be used to process 144 samples (72 per reagent cartridge) using the Cellfree500, Complex400, and the Complex400_OBL protocols.
FAQ ID -2935
What sample volumes can be processed using the QIAsymphony DSP Virus/Pathogen protocols?

The sample volume depends on the QIAsymphony DSP Virus/Pathogen protocols:

  • Cellfree200 — 200 µl
  • Cellfree500 — 500 µl
  • Cellfree1000 — 1000 µl
  • Complex200 and Complex200_OBL — 200 µl
  • Complex400 and Complex400_OBL — 400 µl
  • Complex800 and Complex800_OBL — 800 µl
FAQ ID -2929
How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
Sample material can be stored at 2–8°C for up to 6 h. For longer storage, samples should be kept at –20°C or –70°C.
FAQ ID -2934
Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
No. The use of internal controls is not mandatory, but is strongly recommended. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).
FAQ ID -2932