To visualize contamination of RNA with genomic DNA, quantitative real-time PCR assays were performed with (+RT) or without (–RT) reverse transcriptase. Unlike kits from other suppliers, the AllPrep DNA/RNA/miRNA Universal Kit delivered high yields of pure RNA from difficult-to-lyse adipose tissue, as demonstrated by c-Jun expression assays.
miR1 expression levels in muscle tissue were investigated using miRNA isolated from the AllPrep DNA/RNA/miRNA Universal Kit and products from the indicated suppliers. The AllPrep DNA/ RNA/miRNA Universal Kit delivered higher yields of miRNA, as illustrated by real-time PCR data.
The AllPrep DNA/RNA/miRNA Universal Kit outperforms products from other suppliers, delivering high yields of DNA of superior quality from a various types of tissue, including difficult-to-lyse adipose tissue.