FastLane Cell RT-PCR Kits use FastLane technology to prepare cell lysates for real-time one-step RT-PCR and multiplex, real-time one-step RT-PCR. The kits accelerate and streamline real-time PCR analysis of cultured cells by eliminating the need for RNA purification and deliver reproducible results in high-throughput analysis, enabling easy processing of 96 or 384-well plates.
With the FastLane Cell SYBR Green Kit, specific transcript detection is guaranteed in real-time PCR analysis of cells when the kit is used in combination with QuantiTect Primer Assays. These are predesigned, genomewide primer sets, available at GeneGlobe.
All types of probe chemistry are compatible with the FastLane Cell Probe Kit, including hydrolysis probes (e.g., TaqMan® or other dual-labeled probes), LightCycler hybridization (FRET) probes and Molecular Beacons. A ready-to-use RT-PCR master mix containing a novel RT-PCR buffer and a hot-start DNA polymerase eliminates the need to optimize probe concentration and other PCR parameters.
FastLane Cell Multiplex Kits are ideal for rapid, high-throughput gene expression analysis, such as validation of siRNA-mediated gene knockdown. Unlike other methods, FastLane Cell Multiplex Kits deliver success in multiplex, real-time one-step RT-PCR at the first attempt (see figure " Multiplex analysis with no optimization steps") due to an optimized master mix containing a novel RT-PCR buffer and a hot-start DNA polymerase. Two kit formats are available: the FastLane Cell Multiplex Kit for cyclers that require ROX dye for fluorescence normalization, and the FastLane Cell Multiplex NR Kit for all other cyclers.