BioSprint DNA Plant Kits

For rapid and economical automated purification of total DNA from plant tissue

Features

  • High-throughput isolation of DNA
  • Maximal flexibility to accommodate challenging sample material
  • Complete removal of contamination and inhibitors
  • No organic extraction or alcohol precipitation
BioSprint 96 DNA Plant Kit (576)

Cat. No. / ID: 941557

For 576 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents
Preparations
576
1536
BioSprint DNA Plant Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

BioSprint DNA Plant Kits provide all reagents and plasticware necessary for automated purification of total DNA from plant tissue using proven magnetic-particle technology on the BioSprint 15 or BioSprint 96.

Performance

DNA can be successfully purified from a variety of plant species and material (see tables “DNA purified from a wide range of plant species using the BioSprint 96”). 

DNA purified from a wide range of plant species using the BioSprint 96

Sample type Amount Yield (µg)
Arabidopsis, fresh leaves 50–120 mg 13–41
Cotton, fresh leaves 50 mg 10–26
Maize, fresh leaves 50 mg 10–41
Pine, fresh needles 100–190 mg 5–18
Rose, fresh new leaves 100–140 mg 40–100
Rose, fresh old leaves 100–140 mg 11–51
Soybean, fresh leaves 50 mg 39–80
Spruce, fresh needles 130–160 mg 5–13
Wheat, fresh leaves 50 mg 8–49
Maize, lyophilized leaves 5–10 mg 10–15.5
Rapeseed, lyophilized leaves 5–10 mg 13.5–23
Sunflower, lyophilized leaves 5–10 mg 57–93
Maize, dried seed powder 20–55 mg 9.8–33.5
Apple 1–6 seeds 5–30
French bean 1 bean 13–55
Grape 3 seeds 18.2–18.8
Linen 1–10 seeds 1.5–38
Lupine 2–7 seeds 18–97
Orange 1 seed 1–35
Rapeseed 1–4 seeds 8.5–47.5
Soybean 1 seed 80–105
Swedish turnip 5–50 seeds 21.5–52
Tomato 15–30 seeds 12–16
Turnip radish 4–18 seeds 11–41
Wheat 3–4 seeds 85–122

Purified DNA is up to 40 kb in size (see figure " Reproducible purification of high-quality DNA"). The DNA is ready to use in a range of downstream applications, including PCR and real-time PCR, genotyping and microsatellite analysis (see figures " High PCR performance of DNA purified using the BioSprint 15 DNA Plant Kit” and " High PCR performance of purified DNA”).

See figures

Principle

BioSprint DNA Plant Kits are used with the BioSprint 15 and BioSprint 96. The kits use magnetic-particle technology for rapid DNA purification from plant tissue. BioSprint instruments control an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube or well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure " Efficient processing of magnetic particles"). Since the instruments transfer magnetic particles instead of liquids, they use minimal amounts of reagents, enabling cost-efficient sample preparation.

See figures

Procedure

BioSprint DNA Plant Kits enable purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to a 5-well strip or S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart " BioSprint DNA Plant procedure"). The kits provide all the necessary reagents and plasticware for sample preparation, and are available in different sizes, allowing cost-efficient use of consumables.

See figures

Applications

BioSprint DNA Plant Kits purify DNA for use in a range of downstream applications, including:

  • PCR
  • Real-time PCR
  • Genotyping
  • Microsatellite analysis

Comparison of BioSprint DNA Plant Kits

Features BioSprint 15 DNA Plant Kit BioSprint 96 DNA Plant Kit
Compatible instrument BioSprint 15

BioSprint 96

Thermo KingFisher Flex

Applications PCR, real-time PCR, genotyping PCR, real-time PCR, genotyping
Elution volume 200 µl 200 µl
For automated processing BioSprint 15 workstation BioSprint 96 workstation
Format 5-tube strip, S-Block 5-tube strip, S-Block
Main sample type Plant samples Plant samples
Processing Automated Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA DNA
Sample amount 10–100 mg 10–100 mg
Technology Magnetic particles Magnetic particles
Time per run or per prep 18 minutes 15 minutes
Yield 5–50 µg 5–20 µg

Supporting data and figures

Resources

Kit Handbooks (1)
Brochures & Guides (1)
Rapid and economical preparation of 1–15 or 1–96 samples using magnetic-particle technology

FAQ

Can the BioSprint instruments be placed in a cool room?

No, the BioSprint Instruments should not be operated in a cool room. Optimal operation temperature for BioSprint is 10-40°C. Operation at 4°C may harm the instruments.

 

FAQ ID -938
Do you have stability data for genomic DNA isolated with the BioSprint Kits?

Yes. We have shown that DNA purified with the BioSprint DNA Kits (e.g., the BioSprint 15 DNA Blood Kit, BioSprint 15 DNA Plant Kit, BioSprint 96 DNA Blood Kit and BioSprint 96 DNA Plant Kit) is stable for at least 2 years when being stored at either 2-8°C or -20°C. Our data indicate however that DNA stability is dependent upon storage conditions. For additional information see also FAQ 518.

 

FAQ ID -1318
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?

Yes, DNA can be isolated from lyophilized plant material on the BioSprint 96 instruments using the BioSprint 96 DNA Plant Kit. We recommend starting with no more than 10 or 30 mg of lyophilized plant, respectively. Optimal starting amounts depend on plant type and need to be determined empirically.

FAQ ID -772
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What are the expected average DNA yields from plant extractions on the BioSprint 96?
Typically, DNA yields obtained on the BioSprint 96 range from 5 to 20 ug of DNA from 10 to 100 mg of fresh/frozen plant material using the BioSprint 96 DNA Plant Kit. Yield depends on sample type, sample collection method, and method of tissue disruption.
FAQ ID -771
How do I perform a DNA precipitation to concentrate my sample?
  • Add 1/10 volume of 3 M Na-Acetate pH 5.2, and 2 to 2.5 volumes of ice-cold 100% ethanol to the DNA sample
  • Mix, and store at -20°C for at least 1 hour to precipitate the DNA
  • Recover the precipitated DNA by centrifugation at full speed in a microcentrifuge for 15-20 minutes
  • Pour off the ethanol and wash the pellet twice with room-temperature 70% ethanol
  • Allow the DNA pellet to air-dry
  • resuspend the DNA in a suitable volume of sterile TE buffer or distilled water
FAQ ID -305
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?

The BioSprint 96 instrument has a magnetic-particle collecting efficiency above 95%. However, to avoid particle carryover from the eluate plate to subsequent applications, you can use a plate magnet while pipetting.

 

FAQ ID -2073
How to quantitate DNA purified using the BioSprint 96?

Measure the absorbance of the eluate obtained on the BioSprint 96 instruments at 260 nm, 280 nm and 320 nm. Carryover of magnetic particles in the eluates may affect the A260 and A280 readings, but should not affect the performance of the DNA in downstream applications. Subtract the absorbance reading obtained at 320 nm from the readings obtained at 260 nm and 280 nm to correct for the presence of residual magnetic particles.

FAQ ID -773
Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?

Yes, the RLT lysate generated during the BioSprint DNA Plant protocol can be stored frozen. Redissolve any precipitates that may have formed during storage by gentle heating and continue with the isolation procedure.

FAQ ID -1248