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BioSprint 96 DNA Blood Kit

For rapid and economical automated purification of DNA from a wide range of samples

  • Complete removal of contamination and inhibitors
  • Purification of high-quality DNA that is ready for use
  • Automated procedure saves time and effort
  • No organic extraction or alcohol precipitation

The BioSprint 96 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 96.

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Cat No./ID: 940054
BioSprint 96 DNA Blood Kit (48)
For 48 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
Cat No./ID: 940057
BioSprint 96 DNA Blood Kit (384)
For 384 preps: Large 96-Rod Covers, 96-Well Microplates MP, S-Blocks, MagAttract Suspension G, buffers and reagents
The BioSprint 96 DNA Blood Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details


The BioSprint 96 DNA Blood Kit enables purification of DNA from a variety of sample types (see table).

Typical yields from a range of sample types
Sample typeAmount of sampleDNA (µg)
Muscle, bovine25 mg13.5 ± 1.5
Heart, bovine25 mg3.4 ± 0.6
Spleen, bovine25 mg59.1 ± 4.8
Lung, bovine25 mg14.7 ± 5.5
Liver, bovine25 mg74.0 ± 22.3
Kidney, bovine25 mg33.5 ± 5.4
Tail, mouse1.2 cm (~25 mg)30.9 ± 4.5
HL-60 cultured cells2 x 106 cells9.6 ± 5.6
Blood, human (5–7 x 106 cells per ml)200 µl4.5–9.0
Blood, bovine200 µl4.4–7.0
Blood, sheep200 µl1.2–8.0
Blood, pig200 µl4.5–9.0
Blood, dog200 µl6.9–16.1
Blood, cat100 µl2.9–6.3
Blood, mouse100 µl1.5–3.0
Blood, rat100 µl1.0–3.0
Dried blood spots, 903 Specimen Collection Paper1 punch (6 mm [1/4 inch] diameter)0.10
Dried blood spots, FTA Card1 punch (6 mm [1/4 inch] diameter)0.05
Buccal swabs1 swab0.8–2.0

Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE.

Purified DNA is up to 50 kb in size (see figure "Purification of high-quality genomic DNA from mouse tail"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure "High PCR performance of DNA purified using the BioSprint 96 DNA Blood Kit").



The BioSprint 96 DNA Blood Kit is used with the BioSprint 96. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 96 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.

Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.

For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 96 DNA Blood Kit). We recommend sealing S-Blocks with Tape Pads during sample processing (not needed for rapid blood protocol).


The BioSprint 96 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:

  • Southern blotting
  • PCR and real-time PCR
  • Genotyping


Applications PCR, real-time PCR, genotyping, blotting
Elution volume Custom
For automated processing BioSprint 96 workstation
Format S-Block, 96-well plate
Main sample type Blood, tissue, cells
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 100–250 µl
Technology Magnetic particles
Time per run or per prep 45 minutes
Yield Varies

Product Resources

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Brochures & Guides (1)
Rapid and economical preparation of 1–15 or 1–96 samples using magnetic-particle technology
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Kit Handbooks (1)
For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 96 workstation
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