For high-throughput isolation of DNA from microbial culture
Breaks down tough microbial samples with bead-beating protocol
High-throughput protocol processes 96 microbial samples in 1.5 hours
Ready-to-use, high-quality DNA for downstream applications
For high-throughput processing of microorganisms, consider DNeasy UltraClean 96 Microbial Kit.
The 96-well format successfully isolates a variety of microorganisms, including bacteria, yeast,
fungal spores and Gram-positive and -negative bacteria. Cells are lysed through a combination of
heat, detergent and mechanical force from specialized beads. Released DNA is bound to a silica
spin plate that is then washed. A certified DNA-free Tris buffer recovers DNA from the plate.
The DNeasy UltraClean 96 Microbial Kit was previously sold by MO BIO as the Ultraclean 96
Microbial DNA Kit.
For 1000 x 50 µl reactions (available in a single tube): QIAGEN OneStep RT-PCR Enzyme Mix (2 x 1 ml), 5x QIAGEN OneStep RT-PCR Buffer (1 x 10 ml), dNTP Mix (2 x 1 ml, 10 mM each), 5x Q-Solution (1 x 10 ml), RNase-Free Water (1 x 40 ml)