C. albicans was added to 5 g of sterile compost samples. Protein was then extracted from those samples. Samples processed using the NoviPure Soil Protein Kit show greater protein extraction compared to the sucrose and phenol methods.
Challenging soil types still yield high amounts of protein using the NoviPure Soil Protein Kit. The kit was used to extract total protein from 5 g samples of sterile soil and sediments spiked with E. coli. To visualize protein results, 20 µl of each sample was processed using a stain-free 1D SDS-PAGE gel. In all cases, protein bands were clear and matched the E. coli culture control.
The NoviPure Soil Protein Kit is successful in extracting high-quantity and quality protein, as shown in 2D gel electrophoresis. E. coli-laced 5 g sterile compost samples were processed with the NoviPure Soil Protein Kit. Results were visualized using silver staining following 2D SDS-PAGE, with a pH range of 3–10, using 20 µl per protein sample. Humic substances did not interfere with visualization of proteins.
Protein was extracted using the NoviPure Soil Protein Kit and analyzed by 2D LC-MS/MS, where total ion chromatogram data reveals base peaks of protein. Visualized here is fraction 4 of the SCX cation exchange. The NoviPure Soil Protein Kit extracted protein from 5 g of sterile compost samples spiked with E. coli. Digestion and peptide enrichment followed using reverse phase ion exchange. Comparing raw spectra results with the SwissProt protein database (March 2013 release), 1076 unique proteins were identified.