Real-time one-step RT-PCR using the FastLane Kit and a QuantiTect Primer Assay for INPP5D (a phosphatase) enabled specific transcript quantification in HeLa, HepG2, and Cos cells, as shown by the single peaks in melting curve analysis.
Using the FastLane Kit, A549 cells in a 384-well plate were analyzed for the expression of Eg5, GAPDH, HMBS, and Rab5. Data show the mean CT value with standard deviation from triplicate wells. (Data kindly provided by Anne Grabner, Cenix BioScience GmbH, Germany.)
Real-time one-step RT-PCR using the FastLane Kit and a QuantiTect Primer Assay for INPP5D (a phosphatase) gave reproducible CT values from a 96-well plate of HeLa ACC cells.
Two low-abundance transcripts, INPP5D (a phosphatase) and SRF (serum response factor), were quantified by real-time one-step RT-PCR. The FastLane Kit provided CT values comparable to those achieved with the RNeasy Mini Kit and QuantiTect SYBR® Green RT-PCR Kit.