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Qproteome Nuclear Protein Kit

For separation of nuclear and nucleic acid binding proteins

  • Nuclear preparations free of cytosolic proteins
  • Greatly reduced sample complexity
  • Highly reproducible fractionation
  • Includes protocols for tissue proteomics

The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells.

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Cat No./ID: 37582
Qproteome Nuclear Protein Kit
For 12 nuclear protein preparations: Buffers, Reagents, Protease Inhibitor Solution, Benzonase
The Qproteome Nuclear Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance
The Qproteome Nuclear Protein Kit provides highly effective and reproducible separation of nuclear proteins as demonstrated using specific marker proteins (see figure "Reproducible, efficient separation of marker proteins"), which remain functionally active (see figure "Isolation of an active transcription factor").
Principle

The Qproteome Nuclear Protein Kit is designed for specific enrichment of nuclear proteins from cultured mammalian cells. Lysis and centrifugation are used to separate the cytosolic fraction (supernatant) from the cell nuclei (pellet). A high-salt buffer allows dissociation of nuclear binding proteins (such as transcription factors) and their removal by diffusion from the nuclei.

Procedure
Cells are lysed and centrifuged to isolate nuclei. After washing, an extraction buffer is added to the nuclei and nucleic acid binding proteins dissociate from DNA and RNA. This soluble fraction is separated from the nuclear pellet by centrifugation. Proteins remaining in the pellet are solubilized using a second extraction buffer (see figure "Nuclear protein fractionation procedure").
Applications

The Qproteome Nuclear Protein Kit delivers a nucleic acid binding protein fraction suitable for a wide range of activity assays.

Specifications

Features
Specifications
Applications SDS-PAGE, mass spectrometry
Binding capacity/yield 200–300 µg
For glycoproteins: which type of glycoproteins n.d
Fractions isolated Three fractions
Sample size 5 x 10e6 cells
Species Mammal
Start material Cell cultures

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