BioSprint 15 DNA Blood Kit
For rapid and economical DNA purification from a wide range of sample types
The BioSprint 15 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 15.
DNA can be successfully purified from a variety of sample types (see table).
Purified DNA is up to 50 kb in size (see figure "Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure "High PCR performance of DNA purified using the BioSprint 15 DNA Blood Kit").
The BioSprint 15 DNA Blood Kit is used with the BioSprint 15. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 15 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 15 DNA Blood Kit).
The BioSprint 15 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:
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