TissueLyser II

For medium- to high-throughput sample disruption for molecular analysis

Features

  • Convenient and secure disruption process
  • Adapter sets optimized for high-throughput disruption
  • Wide range of accessories available
  • Reproducible results with difficult-to-lyse tissues
  • Front-end solution for QIAGEN automation
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TissueLyser II

Cat. No. / ID: 85300

Bead mill, 100–120/220–240 V, 50/60 Hz; requires the TissueLyser Adapter Set 2 x 24 or TissueLyser Adapter Set 2 x 96 (available separately)
A$13,728.00
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The TissueLyser II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The TissueLyser II simultaneously disrupts multiple biological samples through high-speed shaking in plastic tubes with stainless steel, tungsten carbide, or glass beads. Using the appropriate adapter set, up to 48 or 192 samples can be processed at the same time. Alternatively, a grinding jar set can be used to process large samples. A range of beads, bead dispensers, and collection microtubes and caps are also available.

All TissueLyser II accessories, including adapter sets, are also compatible with the TissueLyser (no longer available).

Performance

The TissueLyser II is well-suited for high-throughput disruption of human, animal, and plant tissues, bacteria, and yeast. Highly reproducible purification of high-quality DNA, RNA, miRNA, and protein is achieved, even with difficult-to-lyse tissues (see figures "Efficient disruption of animal tissues", "Reproducible RNA purification from plant tissues", " Reproducible DNA purification from animal tissue", " Purification of intact protein from animal tissue", " Reproducible RNA purification from Gram-positive bacteria", and " Reliable detection of miRNAs").
See figures

Principle

Genotyping, gene expression, and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA, and protein. Effortless disruption with the TissueLyser II system is achieved through high-speed shaking with beads, which beat and grind samples. The TissueLyser II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").

The TissueLyser II is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA, and protein. The TissueLyser II complements QIAGEN automation for high-throughput sample preparation and analysis (see table "QIAGEN high-throughput automation"), such as the QIAsymphony SP. This easy-to-use instrument automates purification of DNA, RNA, and protein from 1–96 samples. The TissueLyser II is also compatible with QIAGEN manual sample preparation kits (see table "QIAGEN purification kits compatible with QIAGEN disruption systems").

For RNA applications, stabilization of fresh tissues in RNAprotect Tissue Reagent prevents RNA degradation during sample handling. For applications requiring purification of DNA, RNA, and protein, these 3 analytes can be immediately stabilized by placing fresh tissues in Allprotect Tissue Reagent.

QIAGEN high-throughput automation
Instrument Purpose Throughput
QIAsymphony SP Purification of DNA, RNA, and protein 1–96 samples per run
BioRobot Universal System Purification of DNA and RNA 96-well format
QIAxcel Electophoretic analysis of DNA fragments and RNA Up to 96 samples per run
QIAgility Reaction setup Up to 384 samples per run
Rotor-Gene Q Real-time PCR and high-resolution melting (HRM) analyses Up to 100 samples per run
PyroMark Q96 MD or ID Methylation and mutation analyses Up to 96 samples per run

 

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified Sample type QIAGEN kit
RNA Easy-to-lyse tissue

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits
RNA All types of tissue RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA Plant tissue RNeasy Plant Mini Kit
RNA Yeast RNeasy Kits
RNA Bacteria RNeasy Protect Bacteria Kits
microRNA All types of tissue miRNeasy Kits
DNA Human tissue QIAamp DNA Kits
DNA Animal tissue DNeasy Blood & Tissue Kits
Protein Tissue Qproteome Mammalian Protein Prep Kit
Phosphoprotein Tissue PhosphoProtein Purification Kit
Glycoprotein Tissue Qproteome Glycoprotein Kits
DNA and RNA Tissue AllPrep DNA/RNA Kits
DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Mini Kit

 

Procedure

TissueLyser Adapter Sets allow processing of up to 2 x 96 samples in collection microtubes or up to 2 x 24 samples in 2 ml microcentrifuge tubes. The adapter sets can be precooled at –80°C if disrupting samples without lysis buffer. Tubes remain securely sealed during disruption to prevent cross-contamination, which is especially important for highly sensitive downstream applications, such as real-time RT-PCR or microarray analysis. Depending on the sample type, disruption is carried out using stainless steel, tungsten carbide, or glass beads. TissueLyser Bead Dispensers are available to conveniently deliver single beads into microcentrifuge tubes or to deliver 96 beads in parallel into collection microtubes. The TissueLyser II can also disrupt large samples when used in combination with Grinding Jar Sets.

Applications

The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications. For next-generation, high-throughput sequencing technologies, the TissueLyser II is the disruption instrument of choice. A wide range of QIAGEN sample purification kits are compatible with the TissueLyser II, and sample purification can be performed manually or can be automated using the QIAcube, QIAsymphony SP, EZ1 Advanced, or BioRobot or BioSprint workstations.

Supporting data and figures

Specifications

FeaturesSpecifications
Disruption principleHigh-speed shaking of samples in 1.2 ml collection tubes or 2 ml microcentrifuge tubes with stainless steel or glass beads
Typical run time15 sec - 2 x 3 minutes at 15–30 Hz
Technical data100–120/220–240 V, 50/60Hz; variable speeds from 3 to 30 Hz (180–1800 oscillations/minute)
Kits compatible with instrumentAll kits for purifying RNA/DNA/Protein
Protocol/main application on this intrumentSample preparation/sample disruption
Throughput2 x 96 collection microtubes (1.2 ml) or 2 x 24 microcentrifuge tubes (2ml)
FeaturesConvenient and secure disruption process. Adapter sets optimized for high-throughput disruption. Wide range of accessories available (e.g.,grinding jar set to process large samples). Reproducible results with difficult-to-lyse tissues. Front-end solution for QIAGEN automation.
TechnologyBead Mill

Resources

Supplementary Protocols (8)
Supplementary protocol using the EZ1 Advanced DNA Investigator Large-Scale Bone Card or the EZ1 Advanced XL DNA Investigator Large-Scale Bone Card
Up to 1 x 109 bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.
Instrument User Manuals (1)
Kit Handbooks (1)
For high-throughput disruption of biological samples

FAQ

How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?

Efficient DNA isolation requires thorough sample disruption and digestion.

Although the QIAamp and DNeasy procedures requires no mechanical disruption of the tissue sample, the lysis time will be reduced if the sample is ground in liquid nitrogen or mechanically homogenized in advance. For mechanical homogenization, a rotor–stator homogenizer, such as the QIAGEN TissueRuptor, or a bead mill, such as the QIAGEN TissueLyser, can be used.

To improve digestion of tough tissue samples, Proteinase K incubation at 56°C can be performed overnight. DNA yields may be improved by increasing the amount of Proteinase K or by adding additional proteinase K after several hours of digestion.  

FAQ ID -374