All lysis steps are carried out in the same 96-well flat-bottom block used for growth of the bacteria. After alkaline lysis of the cells, the lysate is neutralized using an optimized buffer, which leads to a virtually clear lysate. Centrifugation or filtration of the lysate is not required. Isopropanol is added in order to optimize the DNA binding conditions, and lysates are transferred to the activated DirectPrep 96 plate where all subsequent steps take place (see flowchart "DirectPrep 96 procedure
"). Plasmid DNA binds to the membrane, which is subsequently washed. After washing, the membrane is dried and the bound plasmid DNA is eluted. The DirectPrep 96 procedure gives typical yields of up to 4 µg DNA from 1.25 ml E. coli
culture grown in LB medium.