Using novel Inhibitor Removal Technology, extract microbial DNA from all soil types with the DNeasy PowerLyzer PowerSoil Kit. Successful qPCR amplification is possible with isolated DNA of the highest level of purity. Processing a wide variety of environmental samples, especially those with a high humic acid content can be done effectively and efficiently with this kit.
The bead tubes contained in this kit are optimized for bead based homogenizers, particularly the PowerLyzer 24 Homogenizer, also available through QIAGEN. Pure microbial genomic DNA can be easily purified from compost, sediment, manure and other environmental samples and successfully amplified by qPCR. A variety of organisms have been detected from samples purified by the DNeasy PowerLyzer PowerSoil Kit, including both Gram (+/-) bacteria (e.g., Bacillus subtilis, Bacillus anthracis), fungi (e.g., yeasts, molds), algae and actinomycetes (e.g., Streptomyces) and nematodes. Genetic material processed is ready for a variety of downstream applications including next-generation sequencing.
Isolation of DNA using the DNeasy PowerLyzer PowerSoil Kit can be automated on the QIAcube Connect.
DNeasy PowerLyzer PowerSoil Kit was formerly sold by MO BIO as PowerLyzer PowerSoil DNA Kit.