text.skipToContent text.skipToNavigation

DNeasy PowerPlant Pro Kit

For the isolation of genomic DNA from plant and seed samples
  • Fast and easy 30-minute protocol isolates DNA without harsh chemicals
  • High-quality DNA from all plant types
  • Efficient processing of difficult plant material yields more DNA per run
  • Effectively removes polyphenolics and polysaccharides with Inhibitor Removal Technology

Looking for a quick and easy solution to purifying genomic DNA? Consider the DNeasy PowerPlant Pro Kit.

DNeasy PowerPlant Pro Kit is superceded by the DNeasy Plant Pro Kit. See new kit

The DNeasy PowerPlant Pro Kit uses mechanical bead beating technology to recover high-quality DNA from tough sample times including cotton leaf and seeds, strawberry leaf, and pine needles without time-consuming or chemical laden methods such as CTAB, chloroform or phenol extraction. Pure isolated DNA is ready for downstream applications, including qPCR, Sanger sequencing and next-generation sequencing through Inhibitor Removal Technology.An optional Phenolic Separation Solution step helps provide pure DNA even from samples with high polyphenolic compounds, such as pine or grape leaves by breaking the bonds between DNA and phenolics. This step prevents DNA loss that would otherwise occur in these samples.

Use the DNeasy PowerPlant Pro Kit in concert with vortex or high velocity bead beating, based on sample needs. Vortex methods work with soft leaf tissue, while high velocity bead beaters, like the PowerLyzer 24 Homogenizer, will break down tougher plant material such as roots, seeds, stems or difficult leaf tissues.

Purification of DNA using the DNeasy PowerPlant Pro Kit can be automated on the QIAcube Connect.


Buy Products

Cat No./ID: 13400-50
DNeasy PowerPlant Pro Kit (50)
$336.00
Order Product
For the isolation of genomic DNA from plant and seed samples
The DNeasy PowerPlant Pro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
Figure 1. High-quality DNA from tough plant samples.
The DNeasy PowerPlant Pro Kit isolates high-quality genomic DNA from plant samples. Using a 1% TAE agarose gel, DNA was isolated from grass (2 µl), strawberry leaf (15 µl), grape leaf (15 µl) and pine needles (1 µl).
1
Figure 2. Greater yields of high molecular weight genomic DNA with the DNeasy PowerPlant Pro Kit.
Using the DNeasy PowerPlant Pro Kit and plant DNA isolation kits from three other suppliers, DNA was isolated from cotton seeds. Isolations were performed per each manufacturer’s protocols. A 1% TAE agarose gel was used to examine a 2% elution of DNA volume.
2
Figure 3. Purified DNA using the DNeasy PowerPlant Pro Kit allows for greater DNA amplification.
Using RuBisCo primers run through qPCR on 1 ng of DNA from pine needles (A) and strawberry leaf (B), both diluted 1:10 and undiluted DNA samples fell within the standard curve (purple lines). The difference between the diluted and undiluted samples was approximately 3 cycles indicating that the samples were freed of PCR inhibitors.
Performance



Specifications

Features
Specifications
Bead size 2.38 mm metal
Binding capacity Up to 20 µg per prep
Processing Bead beating
Sample types Processed plants and seeds
Storage temperature RNase A should be stored at 4°C The other kit reagents and components should be stored at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 30 minutes

Product Resources

fragment fix placeholder

Customers who bought these products also bought
Privacy Policy Sitemap Trademarks & Disclaimers