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Every step of your mRNA sequencing, quality control and analysis is designed for success
Our mRNA NGS service enriches for poly-A tailed transcripts to increase the sequencing depth for coding mRNAs, which improves the sensitivity to mRNAs expressed at low levels. In addition, the library preparation retains information about which of the two DNA strands was used to transcribe a given RNA. This information provides increased confidence in transcript annotation and enables detection of antisense transcript expression.

During data analysis, mitochondrial poly-A tailed transcripts are filtered, as they are considered to be high abundance sequences.

mRNA sequencing is recommended for discovery work and especially for differential expression analysis. Paired-end sequencing increases the mapping percentage to poorly annotated genomes and makes it possible to identify splice variants with much higher confidence. However, if differential gene expression is the primary goal of your project, we recommend single-end sequencing.

Learn more about these RNA Sequencing Services

  • mRNA NGS Seq Service
    Library preparation with poly-A enrichment, sequencing, quality control, data analysis and report.
  • miRNA NGS Seq Service
    Library preparation using the QIAseq miRNA Library Kit (with integrated unique molecular indices, UMIs), sequencing, quality control, data analysis and report.
  • Biofluids miRNA NGS Seq Service
    Library preparation from biofluid using the QIAseq miRNA Library Kit and protocols for the specific fluid, sequencing, quality control, data analysis and report.
  • Targeted RNA Seq Service
    Digital RNA sequencing for gene expression profiling using QIAseq Targeted RNA Panels.
  • Whole Transcriptome Seq Service
    Whole transcriptome preparation, sequencing, quality control, data analysis and report.
  • UPX 3′ Transcriptome Seq Service
    QIAseq UPX 3′ Transcriptome library preparation using integrated unique molecular index (UMI) technology, sequencing, quality control, data analysis and report.

 

Contact Genomic Services to learn more about the options available to you and consult with experts about solutions for your projects.

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