The Pyrogram shows the mutation (nucleotide 34, indicated with an arrow) with the "Sequence to Analyze" GNTGRCGTAGGYA targeting base 2 in codon 12 (nucleotide 35). The yellow color indicates that this sequence is unexpected and needs to be checked.
The mutation GGT → AGT was reanalyzed with the "Sequence to Analyze" NGTGRCGTAGGYA targeting base 1 in codon 12 (nucleotide 34). With this new targeted sequence to analyze, the software rates the assay as one of good quality, confirming the quantification of the mutation.
The components of the PyroMark Q24 MDx System are designed to make the Pyrosequencing workflow straightforward and efficient. Each step, from assay design to PCR amplification and preparation of sequencing templates, is supported by software, kits, reagents, and sample prep instrumentation optimized for Pyrosequencing.
Though small in size, the PyroMark Q24 MDx manages all steps necessary to rapidly analyze up to 24 samples. Simply load samples and reagents, upload the run file, and walk away. The PyroMark Q24 MDx dispenses reagents and nucleotides to each well with precision. Light signals emitted are detected by 24 CCD sensors — one sensor per well — thereby eliminating signal crossover.
The PyroMark Q24 MDx is designed as a stand-alone instrument, which makes it easy to place anywhere in the lab. Data are stored on the instrument hard drive and can be viewed on the instrument screen during a run. Additionally, all files are stored on the supplied USB stick, giving the user the flexibility to analyze data on any computer with PyroMark Q24 MDx Software installed.
The PyroMark Q24 MDx Vacuum Workstation enables conversion of PCR products into the single-stranded DNA needed as template for Pyrosequencing. Exposure of the PCR amplicons to a series of optimized solutions denatures and washes the DNA. This process is carried out for 24 samples in parallel and takes only a few minutes.
The PyroMark Q24 MDx combines ease of use, analysis versatility, and superior detection sensitivity to meet the needs of your diagnostic work. For research purposes, we also offer the PyroMark Q96 ID and PyroMark Q96 MD that can process up to 96 samples in a single run. The PyroMark Q96 MD can additionally be automated to run ten 96-well plates.