EpiTect Methyl II PCR Assays and Arrays provide gene methylation status as percentage unmethylated (UM) and percentage methylated (M) fraction of input DNA. In these examples, the horizontal bar represents the targeted region of a gene from one genome. Biological samples usually contain many genomes derived from many cell types; here, five such genomes are depicted. Light and dark circles represent unmethylated and methylated CpG sites, respectively. In example 2, the targeted region of a gene has two or more methylated CpG sites in two out of five genomes. Thus, the EpiTect Methyl II PCR Assay data reveal that this gene is 60% unmethylated and 40% methylated.
EpiTect Methyl II PCR Array and Illumina Infinium Human Methylation 27 BeadChip assays were performed on MCF-7 cells. A representative analysis of 22 genes is shown. For better comparison, results from the EpiTect Methyl PCR Array were converted to averaged beta values, in which 0 means completely unmethylated and 1 means completely methylated.