Investigator DIPplex Kit

For multiplex amplification of 30 deletion/insertion polymorphisms plus Amelogenin

Features

  • Unique kit featuring alternative markers for human identification
  • Highly suited for degraded DNA thanks to ~160 bp amplicon length
  • Suitable for disaster victim identification and anthropological studies
  • Easy interpretation with dedicated freeware DIPSorter
undefined

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Investigator DIPplex Kit (100)

Cat. No. / ID: 384015

Primer Mix, Reaction Mix, Multi Taq2 Polymerase, Control DNA, DNA Size Standard 550 (BTO), Allelic Ladder DIPplex, and Nuclease-Free Water
€1,837.00
Add to cart
The Investigator DIPplex Kit is intended for molecular biology applications in human identity testing. This product is not intended for the diagnosis, prevention, or treatment of a disease. USA and Canada: Not for use in, or in preparation for, legal proceedings, including parentage determination.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The Investigator DIPplex Kit enables the amplification of 30 deletion/insertion polymorphisms (also known as INDELs) plus Amelogenin. This provides an equally sensitive alternative to STR- and SNP-based human identification. The kit is designed to analyze highly degraded DNA, thanks to the maximum amplicon size of just ~160 bp. The genetic analysis is calibrated with BT5 Matrix, an optimized dye technology.

Performance

The Investigator DIPplex Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode), and the ABI PRISM 310, ABI PRISM 3100, and Applied Biosystems 3130 Genetic Analyzers. 

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations demonstrated robust and balanced results with 0.2–0.5 ng, and reliable results with <0.1 ng DNA.

The Investigator DIPplex Kit has significant advantages over standard STR- and SNP-based DNA profile generation, including the absence of stutter peaks and the very short amplicons (under 160 bp).

Principle

Human identification is commonly based on the analysis of short tandem repeats (STRs) or single nucleotide polymorphisms (SNPs), depending on the demands of an examination or on the sample quality. The Investigator DIPplex Kit uses an alternative means of human identification by allowing the analysis of biallelic deletion/insertion polymorphisms (DIPs, also called INDELs).

Procedure

The 30 biallelic DIP loci plus Amelogenin are simultaneously amplified in a single PCR and labeled with four dyes. See the table "Technical specifications" for more information.

Technical specifications
Volume per amplification reaction25 μl
MatrixBT5
Fluorescence labels6-FAM, BTG, BTY, BTR, BTO
Genetic analyzersABI PRISM 310, ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysisApplied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software

Applications

The Investigator DIPplex Kit is for generation of DNA profiles from forensic stains, hair, and bone samples, and it is highly suitable for:

  • Disaster victim identification
  • Anthropological studies
  • Mixed stain analysis
  • Other human identity testing applications

Resources

Template Files (6)
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Technical Information (4)

Enjoy success at first try with high performance QIAGEN Multiplex STR amplification and instrumentation

The most comprehensive and versatile coverage of current international STR standards


Quality initiatives for human identity testing and forensics 
Quick-Start Protocols (1)

FAQ

3196 - What is the DIPsorter Freeware?

DIPSorter Freeware has been developed as a utility application for the Investigator DIPplex Kit. Its function is to re-sort genotype data of biallelic deletion or insertion markers — the so-called DIPs.

DIPSorter Freeware enables the import of Genotype Table text files exported from GeneMapper® ID, GeneMapper ID-X, or Genotyper® software and displays them as sorted genotype tables. Generated data can be printed and exported to *CSV and *PDF files.

3194 - What DNA fragment sizes are required for the Investigator DIPlex kit?

The Investigator DIPlex kit produces short amplicon lengths (max. 150 for 30 DIPs) so that DIPplex can be used for identification of very challenging samples (highly degraded).To get a full DIPplex profile, you need DNA fragments of at least 160 bp. If your sample contains fragments of max.75 bp, you will not get any positive results. Usually, degraded samples show varying average fragment sizes. Therefore, a positive result is dependent on the quality of each sample.

3195 - Where can I find the Population Data for the Investigator DIPlex kit?
The Population data excel file can be found on the “Resources tab” on the product webpage.
3198 - What is the optimal DNA concentration for the Investigator DIPlex Kit?
The optimum DNA input range is between 200-500 pg. The quoted detection limit is 100 pg (full profile with one assay), but reliable results can be obtained with less then 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.