EZ2 Connect Fx

For end-to-end automation of nucleic acid extraction in human identification applications, from reagent setup to elution

Features

  • Purify DNA and RNA from up to 24 samples in around 20 minutes (288 samples/day)
  • Easily access dedicated human ID and forensics protocols with an intuitive touchscreen interface
  • Get maximal process safety thanks to sample tracking with an onboard camera and sample recovery functions
  • Achieve reproducibility with ready-to-use, prefilled forensic grade (ISO 18385) cartridges
  • Increase lab throughput by remotely monitoring your samples with LIMS and QIAsphere connectivity
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EZ2 Connect Fx System

Cat. No. / ID: 9003220

Benchtop instrument for automated isolation of nucleic acids from up to 24 samples in parallel, using sealed prefilled cartridges; includes 2x EZ2 Connect racks (EZ2 Connect Fx Tip Rack and the EZ2 Conect Fx Tip Rack – Flip Cap Tubes), EZ2 Connect Fx Cartridge Rack and 1-year warranty on parts and labor.
The EZ2 Connect is compatible with EZ1 Kits and EZ1&2 Kits (coming soon).
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual.
The EZ2 Connect Fx is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

Meet your lab’s need for increased throughput, reproducibility and flexibility with the EZ2 Connect Fx. This benchtop instrument automates processing of up to 24 samples at a time in around 20 minutes using the EZ1 Advanced XL magnetic-bead purification technology you know and trust. Easily access a variety of dedicated human ID and forensics protocols at the push of a button and isolate high-quality DNA for numerous downstream applications from a wide range of sample types, such as cigarette butts, saliva, blood, touch samples, bone and semen. Benefit from the EZ2 Connect Fx’s flexibility in sample input and elution volumes, with protocols allowing for up 1000 µl of input lysate and elution volumes ranging from 20 µl – 200 µl. Plus, with its remote connectivity feature, you can monitor your runs and manage your instrument even from outside the lab.

Performance

The EZ2 Connect Fx helps labs processing forensic samples attain a whole new level of flexibility and convenience by fully automating nucleic acid extraction from a wide variety of sample types. Boost the speed and reproducibility of your experiments by drastically reducing manual pipetting steps and replacing them with EZ2 Connect Fx’s prefilled-reagent-cartridge system. Increase your lab throughput by processing up to 24 samples at the same time. The EZ2 Connect Fx is also compatible with LIMS and QIAsphere so you can monitor your runs and manage your instrument remotely. Simply install the QIAsphere app on your preferred tablet or smartphone or by accessing the QIAsphere web app through your laptop or desktop browser. The EZ2 Connect Fx provides human ID-tailored protocols vetted on the EZ1 Advanced XL, as well as new protocols for reduced elution volumes and large working volumes. Results from equivalence testing show that the EZ2 Connect Fx provides excellent DNA yields across a range of input sample dilutions, similar to the EZ1 Advanced XL (see figure  Same excellent DNA yield when compared with the EZ1 Advanced XL). In addition, the EZ2 Connect Fx performs consistently across a range of elution volumes, including down to 20 µl (see figure  Consistent results for even very low elution volumes).

See figures

Principle

The EZ2 Connect Fx uses a piercing mechanism to open prefilled reagent cartridges. It then aspirates and dispenses samples and reagents with a 24-channel pipettor head. If heating is required by the protocol, an on-board heating system controls the temperature of the liquids. Nucleic acid binding, washing and elution are all performed automatically with in-tip magnetic bead technology in around 20 minutes. This principle is based on the proven technology of the EZ1 Advanced XL (see figure  Built from proven EZ1 technology).

See figures

Procedure

Switch on the instrument and login using the user management system. Following instructions on the touchscreen display, the user loads labware and samples onto the EZ2 Connect Fx worktable, chooses a preinstalled protocol and starts a run. During the run, the instrument purifies the lysate by binding, washing and eluting the nucleic acids, while the user can leave the lab to focus on other tasks. Once the run is over, the QIAsphere App or LIMS integrated into EZ2 Connect Fx notifies the user that the run is completed, and the user may return to the machine to collect their purified nucleic acids (see figure  Three simple steps to isolate DNA and RNA).

See figures

Applications

Nucleic acids purified using the EZ2 Connect Fx and the appropriate QIAGEN Kits can be used for human ID and forensic downstream analysis such as PCR, qPCR, next-generation sequencing (NGS) or digital PCR (dPCR) (see figure  One instrument, numerous applications).

See figures

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsAutomated nucleic acid purification
TechnologyMagnetic-particle technology
Instrument dimensions57.5 cm (22.6 in) x 72 cm (28.35 in) x 56 cm (22 in) (H x W x D)
Throughput1-24 samples
Sample typesForensic samples: Trace samples, blood, semen, saliva, bones and hair
Protocols/main application on this instrumentTrace, Tip Dance, Large Volume, Large Scale Bone and Normalization
Pipetting range20 µL – 1000 µL
FeaturesOn-board pipetting, heating function, automated reagent-cartridge piercing, UV-LED decontamination, remote connectivity, touchscreen display, camera

Service Plans

EZ2 Connect, Full Agreement

Cat. No. / ID: 9245570

On-site EZ2 Connect instrument repair, including travel, labor and parts, for a period of one year. Response time of two business days. Includes one Preventive Maintenance or Inspection Service during the Full Agreement period.
EZ2 Connect, Core Agreement

Cat. No. / ID: 9245569

One on-site EZ2 Connect instrument repair service and one Preventive Maintenance or Inspection Service, including travel, labor and parts, for a period of one year. Response time of five business days. Includes a 10% discount on additional repair visits during the Core Agreement period.