PAXgene 96 Blood RNA Kit

For high-throughput purification of cellular RNA from whole blood

Features

  • Parallel purification of RNA from 96 samples
  • Standardized sample processing prior to analysis
  • No organic extraction or precipitation
  • Isolation of high-quality cellular RNA

Products

For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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PAXgene 96 Blood RNA Kit (4)

Cat. No. / ID: 762331

4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.
€4,259.00
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PAXgene 96 Incubator Block

Cat. No. / ID: 9238279

Block for denaturation of eluates in PAXgene 96 procedures
€289.00
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Product Details

The PAXgene 96 Blood RNA Kit is for high-throughput purification of cellular RNA in a manual format. Used with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA to support high-throughput projects, such as clinical research studies.

Performance

The PAXgene 96 Blood RNA Kit allows high-throughput isolation of total RNA from human whole blood. Together with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165), it provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA. The robust PAXgene 96 Blood RNA procedure is highly reproducible and typically yields between 4 and 20 µg of RNA from 2.5 ml human whole blood with minimal risk of sample-to-sample cross contamination. PAXgene Blood RNA Tubes contain a proprietary blend of reagents that bring about immediate stabilization of RNA. This blend prevents the drastic changes in the cellular RNA expression profiles that normally take place in vitro, after blood collection (see figures " RNA isolation from blood using standard methods", " Stabilization of gene expression", and " Detection of tyrosinase following blood storage"). The high-throughput procedure is simple, fast, and efficient, and processing 96 samples in parallel provides significant time savings in your clinical research studies.

See figures

Principle

In the PAXgene 96 Blood RNA System, blood is collected directly into PAXgene Blood RNA Tubes, and RNA is isolated using the PAXgene 96 Blood RNA Kit. PAXgene Blood RNA Tubes contain a proprietary blend of reagents that bring about immediate stabilization of RNA. Blood samples can be stored in the PAXgene Blood RNA Tubes for up to 5 days at room temperature. RNA is then isolated using silica membrane technology supplied in the PAXgene 96 Blood RNA Kit. The resulting RNA accurately represents the expression profile in vivo and is suitable for use in a range of downstream applications.

Procedure

Blood samples (2.5 ml) are collected in PAXgene Blood RNA Tubes (available from BD, cat. no. 762165), and may be stored or transported at room temperature if desired. In the PAXgene 96 Blood RNA procedure, RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate (see flowchart " PAXgene 96 Blood RNA procedure"). Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.

See figures

Applications

The RNA isolated from human whole blood is ready for use in a wide range of downstream applications, including:

  • Nucleic acid amplification technologies, such as RT-PCR and NASBA
  • Expression-array and expression-chip analysis
  • Quantitative RT-PCR, including TaqMan technology
  • cDNA synthesis
  • RNase and S1 nuclease protection
  • Northern, dot, and slot blot analysis
  • Primer extension

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, real-time PCR, qPCR, NASBA, cDNA synthesis
Time per run or per prep<1.5 hours
Elution volume45–60 µl
Main sample typeWhole blood
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinCellular RNA
Sample amount2.5 ml
StabilizationYes
CE/FDA/IVD compatibleResearch use only
Format96-well plate
TechnologySilica technology
ProcessingManual (centrifugation or vacuum)
Yield4–20 µg

Resources

Brochures & Guides (4)
For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

 

For high-throughput purification of cellular RNA from whole blood

 

Kit Handbooks (1)
For isolation of cellular RNA from whole blood Important: To be used only in conjunction with PAXgene Blood RNA tubes

FAQ

Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
What kits does QIAGEN offer to extract RNA from whole blood?

Several kit options are available for this application. We recommend using the PAXgene Blood RNA System, which enables the collection, stabilization and transportation of 2.5 ml human whole blood samples, and subsequent rapid and efficient isolation of cellular RNA.

Other products for the isolation of RNA from whole human blood are the QIAamp RNA Blood Mini Kit and the RNeasy Midi Kit for processing up to 1.5 ml and 10 ml human whole blood, respectively.

FAQ ID -304
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
Yes, buffer RDD of the RNase-Free DNase Set will still work. Please make sure that the buffer is thawed completely without any precipitates before using it. If precipitates are visible, the buffer should be slightly heated.
-20
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
The PAXgene 96 Blood RNA Kit well plate purification chemistry was designed to link to the PAXgene Blood RNA Tubes.  Therefore, one PAXgene Blood RNA Tube can be processed per well on the 96-well plate for 96 tubes total per plate.
FAQ ID -2485
Can the PAXgene 96 Blood RNA Kit be used for diagnostic or prognostic procedures?
No. The product is ‘For Research Use.’ Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
FAQ ID - 3471
What is the PAXgene 96 filter plate?
The PAXgene 96 filter plate is a special device that filters contaminants and generates a cleared lysate during centrifugation.
FAQ ID -2486
What additional equipment is needed to purify RNA using the PAXgene 96 Blood RNA Kit?

The PAXgene 96 Blood RNA Kit uses a combination of vacuum and centrifugation to isolate RNA. The vacuum manifold required is the QIAGEN QIAvac 96 vacuum manifold, catalog # 19504. The suggested centrifuge is manufactured by Sigma and sold by QIAGEN, catalog # 81010, as is the additional plate rotor for spinning 2 x 96 plates, catalog # 81031. Additionally, the final heating step of the protocol utilizes an alloy block, the PAXgene 96 Incubation block, catalog # 9238279. Please contact QIAGEN technical service (www.qiagen.com) for more information.

FAQ ID - 3474