Ultra-Clean Production processes combined with nucleic acid depletion ensure potential contamination with residual DNA from expression host or other sources is reduced to a minimum. All components are tested for bacterial and fungal DNA, using generic PCR Assays for 16S and 18S rRNA coding sequences. This facilitates accurate results in microbial testing, quality control procedures or microbiome / metagenome analysis and sequencing (See: " Sensitive bacterial and fungal amplification"). Low biomass sample testing, in particular, benefits from ultra clean reagents and with improved inhibitor resistance and minimal GC-content bias ensures accurate results (See: "Superior inhibitor resistance..." and " Amplify high and low GC…").
The enzyme’s proofreading feature provides a significantly improved fidelity, 70 times higher than the standard Taq polymerases it was compared against (See: "Premium fidelity").
Both the polymerase activity and the exonuclease activity are stringently controlled by specific antibodies, thus preventing premature activity or damage and nonspecific annealing of template and primers during reaction setup. The kit creates blunt end products and is perfectly suited for procedures requiring high-fidelity PCR such as cloning, site directed mutagenesis, overlap extension PCR or analysis of gene editing experiments.
The capability of low degree multiplexing and amplification of long targets up to 10 kb also makes it a perfect choice for amplicon sequencing analysis. Room temperature set-up, integrated visual pipetting and gel loading dyes further improve efficiency in genotyping and genetic testing workflows.