QIAvac Vacuum Systems

For vacuum-driven processing of columns and plates

Features

  • Reduces sample handling and accelerates processing
  • Allows parallel sample processing
QIAvac 24 Plus

Cat. No. / ID: 19413

Vacuum manifold for processing 1–24 spin columns: includes QIAvac 24 Plus Vacuum Manifold, Luer Plugs, Quick Couplings
€304.00
producttypeaccessories
Vacuum manifold
Vacuum flow-through system
for
24 spin columns
Mega/Giga columns
96-well plates
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Product Details

QIAGEN QIAvac vacuum systems enable fast and efficient vacuum processing of up to 24 spin columns using the QIAvac 24 Plus, of 96-well plates using the QIAvac 96 and up to 6 HiSpeed Mega or Giga Columns using the QIAvac HiSpeed LS Vacuum.


For optimal results, we recommend using the QIAvac 24 Plus and QIAvac 96 together with the QIAvac Connecting System and a Vacuum Pump. The QIAvac 24 Plus must be used together with the QIAvac Accessories. For optimal results, we recommend using the QIAvac HiSpeed LS together with a Vacuum Pump.

Performance

Vacuum-driven liquid processing is faster than centrifugation and still gives high, reproducible yields (see figure  "High, reproducible yields of genomic DNA").

See figures

Principle

QIAvac vacuum systems are for vacuum purification using QIAGEN spin kits. Vacuum-driven liquid processing facilitates sample handling and reduces hands-on preparation time by eliminating the tedious loading and unloading of spin columns into a centrifuge and subsequent removal of flow-through during binding and washing procedures (see flowchart  "Vacuum-driven liquid processing on vacuum manifolds"). Preventing cross-contamination is especially critical when purifying nucleic acids from sensitive samples. To overcome this, QIAGEN columns are inserted into disposable VacConnectors, preventing direct contact of the column with the manifold. VacValves ensure constant vacuum pressure. With the QIAvac HiSpeed LS, the sample flow-through, containing possible infectious material, is collected in separate waste bottles. In combination with the QIAvac Connecting System (vacuum connecting system) (see figure  "An integrated system for various applications"), the QIAvac 24 Plus can be used as a flow-through system. The sample flow-through, containing possible infectious material, is collected in a separate waste bottle.


For vacuum processing in 96-well format, the 96-well purification plate sits in the QIAvac 96 top plate, allowing samples and buffers to be efficiently drawn through the wells. The QIAvac 96 base holds an additional module, a waste tray, or collection vessels such as Collection Microtubes or a 96-well microplate during processing. This allows samples to be drawn from one module to the next, avoiding time-consuming labeling and transfer steps.

See figures

Procedure

Simply insert 1–6 columns into the QIAvac HiSpeed LS or 1–24 columns into the QIAvac 24 Plus, add the samples or buffers and turn on the vacuum source (see table “Examples of plasmid kits that can be used with the QIAvac 24 Plus”). For high-throughput vacuum processing using the QIAvac 96, place the waste tray inside the QIAvac base, then place the QIAvac 96 top plate squarely over the QIAvac base. Simply place a QIAGEN 96-well purification plate in the QIAvac 96 top plate, making sure that the plate is seated tightly and turn on the vacuum source. Samples and buffers are drawn efficiently through the columns or wells, facilitating multiple loadings of large sample volumes and eliminating the need to discard flow-through.


Examples of plasmid kits that can be used with the QIAvac 24 Plus

Vacuum manifold Kit Number of preps
QIAvac 24 Plus CompactPrep Plasmid Midi Kit Up to 24
CompactPrep Plasmid Maxi Kit
CompactPrep Plasmid Maxi Kit Up to 24
QIAGEN Plasmid Plus Maxi Kit
QIAGEN Plasmid Plus Plasmid Mega Kit Up to 12
QIAGEN Plasmid Plus Giga Kit Up to 12

Applications

QIAvac Vacuum Systems enable fast and efficient vacuum processing of QIAGEN columns and 96-well plates and are ideally suited to laboratory research. Vacuum-driven liquid processing is an attractive alternative to centrifugation that minimizes the hands-on time needed for sample preparation.

Supporting data and figures

Resources

Kit Handbooks (3)
For vacuum processing of QIAGEN spin columns  
For QIAvac 24, QIAvac 6S, QIAvac 96, Vacuum Regulator
Brochures & Guides (1)
Supplementary Protocols (4)
The two protocols given below are for the use of the Ni-NTA Superflow 96 BioRobot® Kit in manual procedures. The kit has been specially designed and optimized for automated 6xHis-tagged protein purification on QIAGEN® BioRobot Systems. For more details of the advantages of BioRobot Systems see the Ni-NTA Superflow 96 BioRobot Kit Handbook supplied with the kit or contact one of the QIAGEN Technical Service Departments or local distributors listed on the last page of the handbook.
User-Developed Protocols (2)
The procedure has been used successfully for isolation of a single-copy, 14.5 kb, binary plasmid, p35S GUS INT, from Agrobacterium tumefaciens strain GV2260 (1).
This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.

FAQ

Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?
It is possible to use a general laboratory vacuum system with the QIAamp DSP Virus Kit. However, this will require validation in-house. The CE-marked QIAamp Kits have been developed, field-tested and validated with the QIAvac 24 Plus vacuum system (QIAvac 24 Plus, QIAvac Connecting System and Vacuum Pump). Using this system with the QIAamp DSP Virus Kit will avoid lengthy validation procedures for the enduser.
FAQ ID -789
What vacuum pressure should be applied using the QIAvac 24 Plus for the QIAamp DSP Virus Kit and Blood Mini Kit Protocols?

When processing samples with the QIAamp DSP DNA Blood Mini Kit and the QIAamp DSP Virus Kit, the vacuum pressure should be below -800 mbar. The QIAvac 24 Plus and the QIAvac Connecting System should be tested according to Appendix B of the QIAvac 24 Plus Handbook before performing a nucleic acid purification procedure.

If you would like to use an equivalent general laboratory vacuum system, make sure that the minimum vacuum pressure is reached. Performance of the QIAamp DSP DNA Blood Mini or Virus Kits in combination with a house-vacuum system has to be validated by the researcher.

FAQ ID -1033
Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?

No. The DirectPrep 96 plate of the DirectPrep 96 Miniprep Kit cannot be securely positioned in the QIAvac 96 vacuum manifold, and the nozzles may not fit into the correct wells below. Furthermore, adapters are needed to position Elution Microtubes or Microplates in the correct height for elution. If the distance of the "receiving" plate to the nozzles is not optimal, splashing and thus cross contamination can occur. Due to these difficulties with fitting, tightness and potential cross contamination, we do not recommend using the QIAvac 96 with the DirectPrep 96 System.

We recommend to use the QIAvac Mulitwell vacuum manifold in combination with the DirectPrep 96 Miniprep Kit.

FAQ ID -722