Puregene Accessories

For purification of archive-quality DNA from a wide range of sample types

Features

Archive-quality DNA for long-term storage
Reproducible DNA purification
Convenient, scalable purification procedure

Useful Resources

(EN) - Gentra Puregene Handbook

Protein Precipitation Solution (350 ml)

Cat. No. / ID: 158912

350 ml Protein Precipitation Solution

€439.00

SolutionEnzymeAccessories

Solution

Enzyme

Puregene Proteinase K

Clotspin Baskets

Type

Protein Precipitation

Glycogen

Cell Lysis

Cell Suspension

DNA Hydration

RBC Lysis

Volume

350 ml

50 ml

Quantity

Add to cart

Product Details

Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.

Principle

Using Gentra Puregene Kits, cells are lysed with an anionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. Finally, the genomic DNA is recovered by precipitation with alcohol and dissolved in hydration solution (1 mM EDTA, 10 mM Tris·Cl pH 7.5). Purified DNA typically has an A₂₆₀/A₂₈₀ ratio between 1.7 and 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.

Procedure

Archive-quality DNA

Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Gentra Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb.

Applications

DNA purified using Puregene Accessories and Gentra Puregene Kits is highly stable and suited for use in a wide range of applications, such as:

DNA archiving
PCR
SNP analysis
Southern blotting

Resources

For purification of archive-quality DNA from human whole blood, bone marrow, buffy coat, buccal cells, body fluids, cultured cells, tissue, mouse tail, yeast, bacteria

Publications

Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy.

Hoyo C; Murtha AP; Schildkraut JM; Jirtle RL; Demark-Wahnefried W; Forman MR; Iversen ES; Kurtzberg J; Overcash F; Huang Z; Murphy SK;

Epigenetics; 2011; 6 (7):928-36 2011 Jul 1 PMID:21636975

FAQ

The exact composition of RBC Lysis Solution is confidential. RBC Lysis Solution is a component of Gentra Puregene Blood Kits. RBC Lysis Solution selectively lyses human red blood cells leaving white blood cells intact. You can order RBC Lysis Solutin separately.

FAQ ID -2808

The composition of DNA Hydration Solution is as follows:

10 mM Tris
1 mM EDTA
pH 7–8

DNA Hydration Solution is included in QIAGEN’s Gentra Puregene Kits for DNA purification and can also be bought separately from QIAGEN.

FAQ ID -2813

The detailed composition of Cell Suspension Solution is confidential. Cell Suspension Solution is a component of the Gentra Puregene Yeast/Bacteria Kit. Essentially, it is an isotonic solution that prevents spheroblasts from bursting. Cell Suspension Solution can be ordered separately.

FAQ ID -2811

The exact composition of Protein Precipitation Solution is confidential. Protein Precipitation Solution is a component of Gentra Puregene Kits for DNA purification. Essentially, this is a high-salt buffer that lowers the solubility of proteins. Protein Precipitation Solution can be ordered separately.

FAQ ID -2810

RNase A (17500 U), catalog number 19101, is 100mg/ml.

RNase A Solution (650 µl), catalog number 158922, and RNase A Solution (5 ml), catalog number 158924, are both 4mg/ml.

The exact composition of Glycogen Solution is confidential. The Glycogen concentration is 20mg/mL. Glycogen Solution is a component of Gentra Puregene Kits for DNA purification. During isopropanol precipitation, Glycogen Solution acts as a nucleic acid carrier and helps to efficiently precipitate small amounts of DNA. In addition, it facilitates visualization of the DNA pellet. Glycogen Solution can be purchased separately.

Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.

FAQ ID -2812

The exact composition of Cell Lysis Solution is confidential. Cell Lysis Solution is a component of Gentra Puregene Kits for DNA purification. It functions by enabling cell lysis. Cell Lysis Solution can be purchased separately.

FAQ ID -2809

The Puregene RNase A and Autopure RNase A solutions both have a concentration of 4 mg/ml.

FAQ ID -3083

Using QIAGEN Genomic-tips

Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.

Using QIAamp DNA Micro kit

Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.

Using Gentra Puregene Reagents

Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.

FAQ ID -618