For purification of archive-quality DNA from a wide range of sample types
- Archive-quality DNA for long-term storage
- Reproducible DNA purification
- Convenient, scalable purification procedure
Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.
Purity of DNA has a significant effect on the accuracy of results obtained in downstream applications. Sensitive downstream applications, such as PCR, demand the use of DNA of the highest quality and molecular weight for reliable results. Gentra Puregene Kits removes contaminants and enzyme inhibitors enabling purification of highly stable DNA well-suited for archiving. Purified DNA is of high quality demonstrated by molecular weights of 100–200 kb.
DNA purified using Puregene Accessories and Gentra Puregene Kits is highly stable and suited for use in a wide range of applications, such as:
RNase A (17500 U), catalog number 19101, is 100mg/ml.
RNase A Solution (650 µl), catalog number 158922, and RNase A Solution (5 ml), catalog number 158924, are both 4mg/ml.
Note: Glycogen Solution does not work in combination with QIAGEN’s silica membrane technologies, e.g., RNeasy, DNeasy, QIAamp, etc. With these technologies, poly-A carrier RNA is used when dealing with low amounts of nucleic acids.
Using QIAGEN Genomic-tips
Protocol for DNA cleanup using Genomic-tips can be found in the 'Special Applications' section of the QIAGEN Genomic DNA Handbook.
Using QIAamp DNA Micro kit
Up to 10ug of gDNA can be cleaned using the cleanup protocol in the QIAamp DNA Micro Handbook.
Using Gentra Puregene Reagents
Gentra Puregene Handbook has protocols for removal of proteins and RNA from purified gDNA in Appendix C and Appendix D respectively.