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TissueRuptor II

For low-throughput sample disruption for molecular analysis
  • Rapid, effective disruption of a range of sample types
  • Disposable probes help to minimize cross-contamination
  • Time savings through use of disposable probes
  • Visual monitoring of disruption using transparent probes
  • Seamless integration with QIAGEN sample technologies
The TissueRuptor II is a handheld rotor-stator homogenizer that provides rapid, efficient and flexible disruption of human, animal and plant samples for a wide range of downstream applications. The TissueRuptor II uses transparent disposable probes, which helps to minimize the risk of cross-contamination and enables visual control of the sample disruption process. Use of a disposable probe also provides time savings as the probe does not have to be cleaned after homogenization of each sample.

The TissueRuptor II has all the same features and functionality as the old TissueRuptor.

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Cat No./ID: 9002756
TissueRuptor II (230 V, 50/60 Hz, EU/CH)
€779.00
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Handheld rotor–stator homogenizer, 230 V, 50/60 Hz (for Europe [excluding UK and Ireland]), 5 TissueRuptor Disposable Probes
Cat No./ID: 990890
TissueRuptor Disposable Probes (25)
€273.00
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25 nonsterile plastic disposable probes for use with the TissueRuptor II (and the old TissueRuptor)
The TissueRuptor II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance
QIAGEN has tested use of the TissueRuptor II in combination with proven QIAGEN sample preparation kits. Sample disruption with the TissueRuptor II ensures maximal yields of nucleic acids and proteins that perform well in downstream applications such as PCR, western blotting and enzyme assays (see figures "Successful purification of phosphoprotein " and "High performance results in PCR"). For most tissues, disruption and homogenization using the TissueRuptor II gives comparable results to traditional rotor–stator homogenization (see figure "Pure RNA with high RIN values"). Effective disruption of tissue samples using the TissueRuptor II allows reproducible purification of high-quality DNA and RNA using QIAGEN nucleic acid purification kits (see figures "Pure RNA with high RIN values," "Reproducible purification of high-quality genomic DNA" and "Efficient disruption and homogenization of animal tissues").
Principle

Genotyping, gene expression and proteomics applications demand effective disruption of biological samples to ensure high yields of DNA, RNA and protein. The TissueRuptor II system delivers thorough and rapid disruption of samples to fully release biomolecules, and also simultaneously homogenizes samples to facilitate subsequent purification procedures using QIAGEN kits (see table).

The TissueRuptor II is an integral part of QIAGEN's complete solution for sample management — from sample collection to purification and analysis of DNA, RNA and protein. Optimized protocols integrate sample disruption with biomolecule purification, enabling a streamlined, efficient workflow. In addition, a range of automated solutions allow purification and analysis of biomolecules at low to high throughputs.

QIAGEN also provides RNAprotect Tissue Reagent (to stabilize RNA) and Allprotect Tissue Reagent (to stabilize DNA, RNA and protein). Tissues collected in these reagents can also be easily disrupted with the TissueRuptor II.

QIAGEN purification kits compatible with QIAGEN disruption systems
Analyte purified Sample type QIAGEN kit
RNA Easy-to-lyse tissue

RNeasy Kits

RNeasy Plus Kits
RNeasy Protect Kits
RNA Fiber-rich tissue RNeasy Fibrous Tissue Kits
RNA All types of tissue RNeasy Lipid Tissue Kits
RNeasy Universal Tissue Kits
RNA Plant tissue RNeasy Plant Mini Kit
RNA Yeast RNeasy Kits
RNA Bacteria RNeasy Protect Bacteria Kits
microRNA All types of tissue miRNeasy Kits
DNA Human tissue QIAamp DNA Kits
DNA Animal tissue DNeasy Blood & Tissue Kits
Protein Tissue Qproteome Mammalian Protein Prep Kit
Phosphoprotein Tissue PhosphoProtein Purification Kit
Glycoprotein Tissue Qproteome Glycoprotein Kits
DNA and RNA Tissue AllPrep DNA/RNA Kits
DNA, RNA, and protein Tissue AllPrep DNA/RNA/Protein Mini Kit

 

Procedure

The TissueRuptor II is a portable device with a TissueRuptor Disposable Probe. The blade of the probe rotates at a high speed, causing simultaneous disruption and homogenization of a sample through a combination of turbulence and mechanical shearing. Samples that can be processed include human, animal and plant tissues (for plant tissues that are more difficult to disrupt, the TissueLyser II is recommended). Disruption at full speed for as little as 30 seconds is usually sufficient to release nucleic acids or proteins from starting material.

After disruption of a sample, the TissueRuptor Disposable Probe can be discarded, and a new probe can be used to disrupt the next sample. Time is saved and cross-contamination is avoided, as there is no need to clean and reuse the same probe. In addition, the TissueRuptor Disposable Probe is transparent, which allows visual monitoring of the sample disruption process.

Applications

The TissueRuptor II enables efficient disruption and homogenization of human, plant and animal samples, including clotted blood. Purification of RNA, DNA, total nucleic acids or protein can then be performed using QIAGEN kits.

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Kit Handbooks (1)
For low-throughput disruption of biological samples using disposable probes
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