Agarose gel (1%) electrophoresis of 40 DNA samples amplified using REPLI-g whole genome amplification and visualized by SYBR® Green staining. The average product length is typically greater than 10 kb.
Primers (arrows) anneal to the template DNA and are extended at 30°C by Phi 29 polymerase, which moves along the DNA template strand, displacing the complementary strand, while becoming a template itself for replication. In contrast to PCR amplification, MDA does not require different temperatures and ends in very long fragments with low mutation rates.
