A 500 bp and a 1000 bp fragment purified using the MinElute Gel Extraction Kit and three different silica-based DNA purification kits from the indicated suppliers. Two microliters of each eluate was loaded onto a 1.5% agarose gel. M: markers.
MinElute spin column in cross section, showing the unique membrane assembly (utility model pending).
Manifold with luer connectors.
For use with QIAvac vacuum manifolds
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
The 3 tracking dyes enable easy optimization of gel electrophoresis times and monitoring of small DNA fragments.
This simple bind–wash–elute procedure ensures greater convenience.