The JCSG Core Suites
For initial screening using an optimized selection of conditions
The JCSG Core Suites are split into 4 screens of 96 unique conditions in either deep-well blocks (1.5 ml per well) or tubes (10 ml), and are the result of analyzing over 500,000 high-throughput crystallization experiments performed at the JCSG. The 384 crystallization conditions that provided the highest hit rates in initial screening were chosen to form the screens.
Obtaining protein crystals requires screening of a wide array of crystallization conditions. Starting with different sets of initial screening conditions, the search for a suitable condition can be narrowed down by further developing conditions centered around a promising condition that provides crystalline forms (i.e., “a hit”). The Core Suites include three different precipitant types (salts, organics, and polymers) used within three screening strategies (see table “Screening strategies”). The 384 crystallization conditions in the JCSG Core Suites offer a rapid and wide sampling of crystallization space. NeXtal Stock Solutions can be used to develop grids around your original hit conditions while working on the improvement of crystal size and quality.
The JCSG Core Suites are higly suited for screening and improving initial crystallization conditions.
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